Site-specific mutagenesis is a powerful tool to study structure and functions of RNA, DNA
and protein. Here we introduce a method which is easy, efficient and does not require …

We determined the localizations of mismatch repair proteins in living Bacillus subtilis cells.
MutS-GFP colocalized with the chromosome in all cells and formed foci in a subset of cells …

Mutator cells that lack the mismatch repair system (MMR−) occur at rates of 10− 5 or less in
laboratory populations started from wild-type cells. We show that after selection for …

Escherichia coli possesses two repair systems to correct A/G mismatches. One is directed by
dam methylation, the other is dependent on micA (mutY)(Modrich, 1991). The micA (mutY) …

Mismatch repair detection (MRD) is an in vivo method that uses a change in bacterial colony
color to detect DNA sequence variation. DNA fragments to be screened for variation are …

__Abstract__ Genomes of living organisms, from unicellular bacteria to multicellular human,
are threatened by a plethora of DNA lesions. It is estimated that there are~ 100,000 DNA …

In contrast to lesions produced within the DNA helix by chemical damage, mismatched base
pairs arise during the course of normal DNA metabolism. Enzyme systems that recognize …

DNA mismatch repair in Escherichia coli has been shown to be involved in two distinct
processes: mutation avoidance, which removes potential mutations arising as replication …

Postsynthesis mismatch repair is an important contributor to mutation avoidance and
genomic stability in bacteria, yeast, and humans. Regulation of its activity would allow …