Development of in vivo-matured porcine oocytes following intracytoplasmic sperm injection

MJ Martin - Biology of Reproduction, 2000 - academic.oup.com
The objective of this study was to assess the development of porcine ova fertilized by
intracytoplasmic sperm injection (ICSI). Allyl trenbolone (Regumate) was used to …

Birth of a piglet derived from an oocyte fertilized by intracytoplasmic sperm injection (ICSI)

T Kolbe, W Holtz - Animal Reproduction Science, 2000 - Elsevier
This report is of a live piglet derived from an embryo produced by intracytoplasmic sperm
injection and carried to term. In vivo-matured oocytes were injected with in vitro-capacitated …

Viable piglets generated from porcine oocytes matured in vitro and fertilized by intracytoplasmic sperm head injection

M Nakai, N Kashiwazaski, A Takizawa… - Biology of …, 2003 - academic.oup.com
Intracytoplasmic sperm injection (ICSI) of a nonmotile cell into the ooplasm for assisted
fertilization is a highly specialized procedure for producing the next generation. The …

In vitro maturation of porcine oocytes using a defined medium and developmental capacity after intracytoplasmic sperm injection

R Kishida, ES Lee, Y Fukui - Theriogenology, 2004 - Elsevier
To establish a defined in vitro maturation culture system for porcine oocytes, we examined
the effects of adding cysteine (Cys) and epidermal growth factor (EGF) to the maturation …

Caprine blastocyst formation following intracytoplasmic sperm injection and defined culture

L Keskintepe, PC Morton, SE Smith, MJ Tucker… - Zygote, 1997 - cambridge.org
Experiments were undertaken to develop intracytoplasmic sperm injection (ICSI) to produce
caprine embryos out of the normal breeding season. Oocytes were obtained from 2–6 mm …

Experiments to improve in vitro fertilization techniques for in vivo-matured porcine oocytes

D Rath - Theriogenology, 1992 - Elsevier
Three experiments were designed for the in vitro fertilization of in vivo matured oocytes
following different sperm treatments: In experiment I, spermatozoa (sperm rich fraction) were …

Effects of oocyte activation and sperm preparation on the development of porcine embryos derived from in vitro-matured oocytes and intracytoplasmic sperm injection

JH Tian, ZH Wu, L Liu, Y Cai, SM Zeng, SE Zhu, GS Liu… - Theriogenology, 2006 - Elsevier
The objective was to determine the effects of various methods of oocyte activation and
sperm pretreatment on development of porcine embryos derived from in vitro-matured …

Blastocyst formation by pig embryos resulting from in-vitro fertilization of oocytes matured in vitro

M Yoshida, Y Ishizaki, H Kawagishi - Reproduction, 1990 - rep.bioscientifica.com
Follicular oocytes collected from prepubertal gilts at a local slaughter house were matured
(36 h), fertilized and developed in vitro. Of 785 embryos, 190 (24%) embryos cleaved to the …

[PDF][PDF] In vitro fertilization and embryo development in pigs

LR Abeydeera - REPRODUCTION-CAMBRIDGE …, 2001 - biosciproceedings.org
Considerable progress has been made in the in vitro production of pig embryos using
improved methods for in vitro maturation(IVM) and fertilization(IVF). Despite the progress …

Development of embryos produced by intracytoplasmic sperm injection of cat oocytes

CE Pope, CA Johnson, MA McRae, GL Keller… - Animal Reproduction …, 1998 - Elsevier
Development of cat oocytes following intracytoplasmic sperm injection (ICSI) and in vitro
fertilization (IVF) was compared in two experiments. Domestic cat donors (used as a model …