Endoplasmic reticulum acyltransferase with prokaryotic substrate preference contributes to triacylglycerol assembly in Chlamydomonas

Y Kim, EL Terng, WR Riekhof… - Proceedings of the …, 2018 - National Acad Sciences
Understanding the unique features of triacylglycerol (TAG) metabolism in microalgae may
be necessary to realize the full potential of these organisms for biofuel and biomaterial …

Characterization of type 2 diacylglycerol acyltransferases in Chlamydomonas reinhardtii reveals their distinct substrate specificities and functions in triacylglycerol …

J Liu, D Han, K Yoon, Q Hu, Y Li - The Plant Journal, 2016 - Wiley Online Library
Diacylglycerol acyltransferases (DGAT s) catalyze a rate‐limiting step of triacylglycerol
(TAG) biosynthesis in higher plants and yeast. The genome of the green alga …

Phospholipid: diacylglycerol acyltransferase is a multifunctional enzyme involved in membrane lipid turnover and degradation while synthesizing triacylglycerol in the …

K Yoon, D Han, Y Li, M Sommerfeld, Q Hu - The Plant Cell, 2012 - academic.oup.com
Many unicellular microalgae produce large amounts (∼ 20 to 50% of cell dry weight) of
triacylglycerols (TAGs) under stress (eg, nutrient starvation and high light), but the synthesis …

TAG, You're it! Chlamydomonas as a reference organism for understanding algal triacylglycerol accumulation

SS Merchant, J Kropat, B Liu, J Shaw… - Current opinion in …, 2012 - Elsevier
Photosynthetic organisms are responsible for converting sunlight into organic matter, and
they are therefore seen as a resource for the renewable fuel industry. Ethanol and esterified …

Functional divergence of diacylglycerol acyltransferases in the unicellular green alga Haematococcus pluvialis

H Ma, X Wu, Z Wei, L Zhao, Z Li, Q Liang… - Journal of …, 2021 - academic.oup.com
Acyl-CoA: diacylglycerol acyltransferase (DGAT) catalyzes the final committed step in
triacylglycerol biosynthesis in eukaryotes. In microalgae, the copy number of DGAT genes is …

Cloning and characterization of a GPAT-like gene from the microalga Lobosphaera incisa (Trebouxiophyceae): overexpression in Chlamydomonas reinhardtii …

U Iskandarov, S Sitnik, N Shtaida, S Didi-Cohen… - Journal of applied …, 2016 - Springer
The oleaginous green microalga Lobosphaera (formerly Parietochloris) incisa accumulates
high amounts of arachidonic-acid-rich triacylglycerols (TAG), in particular under conditions …

Galactolipid DGDG and betaine lipid DGTS direct de novo synthesized linolenate into triacylglycerol in a stress-induced starchless mutant of Chlamydomonas …

M Yang, F Kong, X Xie, P Wu, Y Chu… - Plant and Cell …, 2020 - academic.oup.com
The increasing demand for triacylglycerol (TAG) enriching polyunsaturated fatty acids
(PUFAs) has led to a surge of interest in microalgal TAG metabolism. Polar membrane lipids …

[HTML][HTML] A chloroplast pathway for the de novo biosynthesis of triacylglycerol in Chlamydomonas reinhardtii

J Fan, C Andre, C Xu - FEBS letters, 2011 - Elsevier
Neutral lipid metabolism has been extensively studied in yeast, plants and mammals. In
contrast, little information is available regarding the biochemical pathway, enzymes and …

[HTML][HTML] Three acyltransferases and nitrogen-responsive regulator are implicated in nitrogen starvation-induced triacylglycerol accumulation in Chlamydomonas

NR Boyle, MD Page, B Liu, IK Blaby, D Casero… - Journal of Biological …, 2012 - ASBMB
Algae have recently gained attention as a potential source for biodiesel; however, much is
still unknown about the biological triggers that cause the production of triacylglycerols. We …

Triacylglycerol accumulates exclusively outside the chloroplast in short-term nitrogen-deprived Chlamydomonas reinhardtii

M Yang, Y Meng, Y Chu, Y Fan, X Cao, S Xue… - Biochimica et Biophysica …, 2018 - Elsevier
In microalgae, triacylglycerol (TAG) biosynthesis occurs by parallel pathways involving both
the chloroplast and endoplasmic reticulum. A better understanding of contribution of each …