The present study examined the penetrability of pig oocytes by frozen-thawed ejaculated
boar spermatozoa, prepared by the pellet method, coincubated in a modified Tris-buffered …

Pig oocytes matured in culture were inseminated with frozen–thawed ejaculated
spermatozoa without preincubation in modified tissue culture medium (TCM) 199. High …

In Exp. 1 pig oocytes matured in vitro were used to evaluate the fertilizability in vitro of frozen
epididymal (4 boars) and ejaculated (3 boars) spermatozoa that were preincubated in …

Pig follicular oocytes cultured in a defined medium for 28–29 h were inseminated in vitro by
epididymal or ejaculated boar spermatozoa that were preincubated in a modified KRB …

The fertilization of pig oocytes following intracytoplasmic injection of freeze-dried
spermatozoa was evaluated. Activation and male pronuclear (MPN) formation were better in …

Various times of in vitro maturation of oocytes, and three methods of separating
spermatozoa from frozen-thawed semen (Percoll density-gradient centrifugation, swim-up …

Boar ejaculated and epididymal spermatozoa were preincubated in modified KRB or the
isolated oviduct and uterine horn of an oestrous sow for 4· 5–5 h at 37° C before introduction …

The objective of this study was to compare the in vitro fertilizing capacity of porcine
spermatozoa from fresh and frozen-thawed semen and frozen-thawed epididymal …

Cumulus-enclosed pig oocytes were matured in vitro, freed from cumulus cells, and
inseminated with frozen–thawed ejaculated spermatozoa in a chemically defined protein …

In the present study, attempts were made to develop a protein-free (PF) in vitro maturation
(IVM) system for pig oocytes and to examine subsequent embryo development after in vitro …