Current solution based proteomic analysis methods are generally based on enzymatic
digestion of a protein mixture followed by separation using multidimensional liquid …

Multidimensional liquid-based separation techniques are described for maximizing the
resolution of the enormous number of peptides generated upon tryptic digestion of …

Multidimensional peptide separation will play an increasingly important role in the drive to
identify and quantitate the proteome. By increasing the peak and load capacity …

Large-scale proteomics often employs two orthogonal separation methods to fractionate
complex peptide mixtures. Fractionation can involve ion exchange separation coupled to …

Shotgun proteomics dominates the field of proteomics. The foundations of the strategy
consist of multiple rounds of peptide separation where chromatography provides the …

In this paper, we present a combined top-down/bottom-up proteomic analysis workflow for
the characterization of proteomic samples. This workflow combines protein fractionation …

Proteomics technologies demonstrate enormous data-gathering capabilities to discover
disease-specific biomarkers in serum, plasma, urine, tissue and other biological samples …

One of the most effective methods for the direct identification of proteins from complex
mixtures without first having to resolve them by polyacrylamide gel electrophoresis is to …

The need for highly resolving separation methods that can be combined with MS has driven
the development of multidimensional LC. Although the concept is not new, Motoyama and …

Multidimensional chromatography coupled to mass spectrometry is an emerging technique
for the analysis of complex protein mixtures. One approach in this general category …