[HTML][HTML] Functional genetic variants revealed by massively parallel precise genome editing
A major challenge in genetics is to identify genetic variants driving natural phenotypic
variation. However, current methods of genetic mapping have limited resolution. To address …
variation. However, current methods of genetic mapping have limited resolution. To address …
Wildtype heterogeneity contributes to clonal variability in genome edited cells
L Westermann, Y Li, B Göcmen, M Niedermoser… - Scientific reports, 2022 - nature.com
Genome editing tools such as CRISPR/Cas9 enable the rapid and precise manipulation of
genomes. CRISPR-based genome editing has greatly simplified the study of gene function …
genomes. CRISPR-based genome editing has greatly simplified the study of gene function …
Genome-wide mapping of mutations at single-nucleotide resolution for protein, metabolic and genome engineering
Improvements in DNA synthesis and sequencing have underpinned comprehensive
assessment of gene function in bacteria and eukaryotes. Genome-wide analyses require …
assessment of gene function in bacteria and eukaryotes. Genome-wide analyses require …
Multiplexed precision genome editing with trackable genomic barcodes in yeast
Our understanding of how genotype controls phenotype is limited by the scale at which we
can precisely alter the genome and assess the phenotypic consequences of each …
can precisely alter the genome and assess the phenotypic consequences of each …
CrispRVariants charts the mutation spectrum of genome engineering experiments
CRISPR-Cas9 and related technologies enable the efficient alteration of genomic DNA at
targeted positions and are widely used in applications ranging from individual gene …
targeted positions and are widely used in applications ranging from individual gene …
Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision
We developed a CRISPR–Cas9-and homology-directed-repair-assisted genome-scale
engineering method named CHAnGE that can rapidly output tens of thousands of specific …
engineering method named CHAnGE that can rapidly output tens of thousands of specific …
BATCH-GE: Batch analysis of Next-Generation Sequencing data for genome editing assessment
A Boel, W Steyaert, N De Rocker, B Menten… - Scientific reports, 2016 - nature.com
Targeted mutagenesis by the CRISPR/Cas9 system is currently revolutionizing genetics.
The ease of this technique has enabled genome engineering in-vitro and in a range of …
The ease of this technique has enabled genome engineering in-vitro and in a range of …
High-fidelity SaCas9 identified by directional screening in human cells
CRISPR-Staphylococcus aureus Cas9 (CRISPR-SaCas9) has been harnessed as an
effective in vivo genome-editing tool to manipulate genomes. However, off-target effects …
effective in vivo genome-editing tool to manipulate genomes. However, off-target effects …
Highly parallel genome variant engineering with CRISPR–Cas9
Understanding the functional effects of DNA sequence variants is of critical importance for
studies of basic biology, evolution, and medical genetics; however, measuring these effects …
studies of basic biology, evolution, and medical genetics; however, measuring these effects …
Whole-organism lineage tracing by combinatorial and cumulative genome editing
INTRODUCTION The developmental path by which a fertilized egg gives rise to the cells of a
multicellular organism is termed the cell lineage. In 1983, John Sulston and colleagues …
multicellular organism is termed the cell lineage. In 1983, John Sulston and colleagues …