CRISPR-Cas9: tool for qualitative and quantitative plant genome editing

A Noman, M Aqeel, S He - Frontiers in plant science, 2016 - frontiersin.org
Recent developments in genome editing techniques have aroused substantial excitement
among agricultural scientists. These techniques offer new opportunities for developing …

Boosting plant genome editing with a versatile CRISPR-Combo system

C Pan, G Li, AA Malzahn, Y Cheng, B Leyson… - Nature Plants, 2022 - nature.com
CRISPR-Cas9, its derived base editors and CRISPR activation systems have greatly aided
genome engineering in plants. However, these systems are mostly used separately, leaving …

[HTML][HTML] Engineering CRISPR/LbCas12a for highly efficient, temperature‐tolerant plant gene editing

P Schindele, H Puchta - Plant Biotechnology Journal, 2020 - ncbi.nlm.nih.gov
Engineering CRISPR/LbCas12a for highly efficient, temperature‐tolerant plant gene editing -
PMC Back to Top Skip to main content NIH NLM Logo Access keys NCBI Homepage MyNCBI …

On-and off-target analyses of CRISPR-Cas12b genome editing systems in rice

F Gurel, Y Wu, C Pan, Y Cheng, G Li, T Zhang… - The CRISPR …, 2023 - liebertpub.com
The CRISPR-associated Cas12b system is the third most efficient CRISPR tool for targeted
genome editing in plants after Cas9 and Cas12a. Although the genome editing ability of …

CRISPR/Cas genome editing: A frontier for transforming precision cassava breeding

BS Juma, C Mweu, M Piero… - African journal of …, 2021 - academicjournals.org
Improved agricultural production of essential crops through advanced breeding is important
for increasing access to nutritious food for the world's rapidly growing population, which is …

CRISPR-based genome editing: advancements and opportunities for rice improvement

WA Zegeye, M Tsegaw, Y Zhang, L Cao - International Journal of …, 2022 - mdpi.com
To increase the potentiality of crop production for future food security, new technologies for
plant breeding are required, including genome editing technology—being one of the most …

Recent advances in genome editing using CRISPR/Cas9

Y Ding, H Li, LL Chen, K Xie - Frontiers in plant science, 2016 - frontiersin.org
The CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9 (CRISPR-
associated nuclease 9) system is a versatile tool for genome engineering that uses a guide …

A simple, flexible and high‐throughput cloning system for plant genome editing via CRISPR‐Cas system

H Kim, ST Kim, J Ryu, MK Choi, J Kweon… - Journal of integrative …, 2016 - Wiley Online Library
CRISPR‐Cas9 system is now widely used to edit a target genome in animals and plants.
Cas9 protein derived from Streptococcus pyogenes (SpCas9) cleaves double‐stranded …

Genome editing in rice: recent advances, challenges, and future implications

R Mishra, RK Joshi, K Zhao - Frontiers in Plant Science, 2018 - frontiersin.org
Rice (Oryza sativa L.) is the major food source for more than three billion people of the
world. In the last few decades, the classical, mutational, and molecular breeding approaches …

Efficient CRISPR/Cas9-based genome editing and its application to conditional genetic analysis in Marchantia polymorpha

SS Sugano, R Nishihama, M Shirakawa, J Takagi… - PloS one, 2018 - journals.plos.org
Marchantia polymorpha is one of the model species of basal land plants. Although
CRISPR/Cas9-based genome editing has already been demonstrated for this plant, the …