Alfalfa (Medicago sativa) is an outcrossing tetraploid legume species widely cultivated in the
world. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR …

The efficiency of the CRISPR/Cas9 genome editing system remains limited in many crops.
Utilizing strong promoters to boost the expression level of Cas9 are commonly used to …

CRISPR/Cas9 system has been widely applied in many plant species to induce mutations in
the genome for studying gene function and improving crops. However, to our knowledge …

Genome editing technology represented by CRISPR-Cas9 had been widely used in many
biological fields such as gene function analysis, gene therapy, and crop improvement …

Conventional breeding techniques for crop improvement are based on hybridization and
selection. However, due to the long breeding cycles of crops and the potentially …

Lack of appropriate methods for delivery of genome-editing reagents is a major barrier to
CRISPR/Cas-mediated genome editing in plants. Agrobacterium-mediated genetic …

The CRISPR/Cas system has rapidly become the preferred tool for genome engineering in
various organisms due to high efficiency, specificity, simplicity and versatility. Currently …

Genome editing through the alteration of nucleotide sequence has already revolutionized
the field of site-directed mutagenesis for a decade. However, research in terms of precision …

Abstracts Processes of traditional trait development in plants depend on genetic variations
derived from spontaneous mutation or artificial random mutagenesis. Limited availability of …

In the process of acquiring mutants mediated by CRISPR/Cas9, plantlets are often
regenerated from both mutated and non-mutated cells in a random manner, which increase …