An Improved Procedure for Agrobacterium-Mediated Transformation of 'Carrizo' Citrange

Y Li, D Tang, Z Liu, J Chen, B Cheng, R Kumar, H Yer… - Plants, 2022 - mdpi.com
Although several protocols for genetic transformation of citrus have been published, it is
highly desirable to further improve its efficiency. Here we report treatments of Agrobacterium …

Efficient transformation and regeneration of transgenic plants in commercial cultivars of Citrus aurantifolia and Citrus sinensis

S Singh, Z Tarannum, S Kokane, DK Ghosh… - Transgenic …, 2023 - Springer
Citrus is one of the major horticultural crops with high economic and nutraceutical value.
Despite the fact that conventional research has developed numerous improved varieties …

Evaluation of parameters affecting Agrobacterium-mediated transformation of citrus

M Dutt, JW Grosser - Plant Cell, Tissue and Organ Culture (PCTOC), 2009 - Springer
An improved protocol for genetic transformation of juvenile explants of Carrizo (Citrus
sinensis Osb.× Poncirus trifoliata L. Raf.), Duncan (Citrus paradisi Macf.), Hamlin (Citrus …

Citrus

V Orbović, JW Grosser - Agrobacterium Protocols Volume 2, 2007 - Springer
Since the initial reports on production of transgenic Citrus via Agrobacterium-mediated
transformation, significant progress has been made, and many steps of this procedure using …

A versatile Agrobacterium-based plant transformation system for genetic engineering of diverse citrus cultivars

MM Dominguez, CS Padilla, KK Mandadi - Frontiers in Plant Science, 2022 - frontiersin.org
Developing an efficient transformation system is vital in genetically engineering recalcitrant
crops, particularly trees. Here, we outline an Agrobacterium tumefaciens-based stable plant …

A Versatile Agrobacterium-Based Transformation System for Genetic Engineering of Diverse Citrus Cultivars

MM Dominguez, CS Padilla, KK Mandadi - bioRxiv, 2022 - biorxiv.org
Developing an efficient transformation system is vital in genetically engineering recalcitrant
crops, particularly trees. Here, we outline an Agrobacterium tumefaciens-based …

High efficiency Agrobacterium-mediated transformation and regeneration of citrus

L Pena, M Cervera, J Juárez, C Ortega, JA Pina… - Plant Science, 1995 - Elsevier
A procedure for increased efficiency of production of transgenic citrus plants was developed
by extending the exposure of the explants to the selection agent and by grafting in vitro the …

An improved procedure for Agrobacterium-mediated transformation of trifoliate orange (Poncirus trifoliata L. Raf.) via indirect organogenesis

X Zou, D Li, X Luo, K Luo, Y Pei - In Vitro Cellular & Developmental Biology …, 2008 - Springer
Highly efficient Agrobacterium-mediated transformation of trifoliate orange (Poncirus
trifoliata (L.) Raf.) was achieved via indirect shoot organogenesis. Stable transformants were …

Citrus transformation using mature tissue explants

V Orbović, A Shankar, ME Peeples, C Hubbard… - … Protocols: Volume 2, 2015 - Springer
Mature tissue protocol for production of transgenic Citrus plants via Agrobacterium-mediated
transformation uses explants derived from branches of mature, fruit-bearing trees. Through …

[HTML][HTML] Agrobacterium-mediated transformation of Citrus sinensis and Citrus limonia epicotyl segments

WAB Almeida, FAA Mourão Filho, BMJ Mendes… - Scientia …, 2003 - SciELO Brasil
Genetic transformation allows the release of improved cultivars with desirable characteristics
in a shorter period of time and therefore may be useful in citrus breeding programs. The …