[PDF][PDF] Effects of a novel co-culture system on development, metabolism and gene expression of bovine embryos produced in vitro

S Rief, F Sinowatz, M Stojkovic… - REPRODUCTION …, 2002 - researchgate.net
Early embryonic development takes place in the oviduct, where the preimplantation embryo
experiences a changing environment during the reproductive cycle (Hunter, 1988; Leese …

Effects of bovine oviduct epithelial cells, fetal calf serum and bovine serum albumin on gene expression in single bovine embryos produced in the synthetic oviduct …

ME Pedersen, ØB Øzdas, W Farstad… - Reproduction …, 2006 - CSIRO Publishing
In this study the synthetic oviduct fluid (SOF) system with bovine oviduct epithelial cell
(BOEC) co-culture is compared with an SOF system with common protein supplements. One …

A single medium supports development of bovine embryos throughout maturation, fertilization and culture

AP Gandhi, M Lane, DK Gardner… - Human …, 2000 - academic.oup.com
Oocytes and embryos are typically exposed sequentially to varying culture media in
standard in-vitro protocols. Expenditures of energy may be required following each medium …

Modifications made to culture medium by bovine oviduct epithelial cells: changes to carbohydrates stimulate bovine embryo development

LJ Edwards, PA Batt, F Gandolfi… - Molecular …, 1997 - Wiley Online Library
Co‐culture remains a common method to support the development of bovine embryos,
derived from IVM/IVF procedures. However, the mechanism by which somatic cells confer …

Effects of protein source and co-culture on bovine embryo development in synthetic oviductal fluid medium

RW Rorie, TD Lester, GF Miller, DW Gliedt, RW McNew - Theriogenology, 1994 - Elsevier
Experiment 1 compared the development of 2-to 4-cell bovine embryos cultured in synthetic
oviductal fluid with 20% fetal calf serum or 3.2% BSA and in the presence of oviductal cells …

A serum-free medium for use in a cumulus cell co-culture system for bovine embryos derived from in vitro maturation and in vitro fertilization

JM Lim, A Rocha, W Hansel - Theriogenology, 1996 - Elsevier
The objective of this study was to develop a serum-free medium for the co-culture of bovine
embryos that would yield a percentage of blastocysts equal to that obtained with fetal bovine …

Developmental competence and metabolism of bovine embryos cultured in semi-defined and defined culture media

RL Krisher, M Lane, BD Bavister - Biology of Reproduction, 1999 - academic.oup.com
Abstract Development of in vitro-produced bovine embryos was studied in 3 two-step culture
media: synthetic oviduct fluid (SOF), Gardner's G1/G2, and control (hamster embryo culture …

[PDF][PDF] Three year results of in vitro production of bovine embryos in serum-poor bovine oviduct conditioned medium. An overview

A Van Langendonckt… - Reproduction …, 1996 - rnd.edpsciences.org
Summary ― This paper presents a synthesis of 3 year results of in vitro production of
bovine embryos in medium previously conditioned by bovine oviduct epithelial cells. In …

Unravelling the needs of singly in vitro-produced bovine embryos: from cumulus cell co-culture to semi-defined, oil-free culture conditions

IGF Goovaerts, J Leroy, A Langbeen… - Reproduction …, 2012 - CSIRO Publishing
Producing bovine in vitro embryos individually is a challenge as it generally leads to
impaired embryo development. Earlier research optimised a single embryo in vitro …

Viability of one-cell bovine embryos cultured in synthetic oviduct fluid medium

KJ McLaughlin, DM McLean, G Stevens, RA Ashman… - Theriogenology, 1990 - Elsevier
Synthetic oviduct fluid medium supplemented with human serum (SOFM-HS) was evaluated
as a culture system for maintaining the viability and in vitro development of one-cell bovine …