DNA Uptake Sequence-Mediated Enhancement of Transformation in Neisseria gonorrhoeae Is Strain Dependent

PM Duffin, HS Seifert - Journal of bacteriology, 2010 - Am Soc Microbiol
Natural transformation is the main means of horizontal genetic exchange in the obligate
human pathogen Neisseria gonorrhoeae. Neisseria spp. have been shown to preferentially …

Genetic transformation of Neisseria gonorrhoeae shows a strand preference

PM Duffin, HS Seifert - FEMS microbiology letters, 2012 - academic.oup.com
Natural transformation is the main means of horizontal genetic exchange in the obligate
human pathogen Neisseria gonorrhoeae. Neisseria spp. have been shown to preferentially …

Formation of Single-Stranded DNA during DNA Transformation of Neisseria gonorrhoeae

MS Chaussee, SA Hill - Journal of bacteriology, 1998 - Am Soc Microbiol
Neisseria gonorrhoeae is naturally competent for DNA transformation. In contrast to other
natural prokaryotic DNA transformation systems, single-stranded donor DNA (ssDNA) has …

Sequence-specific DNA uptake in transformation of Neisseria gonorrhoeae

JF Graves, GD Biswas, PF Sparling - Journal of bacteriology, 1982 - Am Soc Microbiol
Piliated, competent gonococci are known to preferentially take up homologous transforming
DNA into the cell. We examined the mechanism for DNA uptake with pFA10, a hybrid 11.5 …

Sequential action of factors involved in natural competence for transformation of Neisseria gonorrhoeae

D Facius, M Fussenegger… - FEMS microbiology letters, 1996 - academic.oup.com
We previously identified and genetically characterized several factors essential for the
natural competence of transformation in Neisseria gonorrhoeae. Here we analyse the …

Natural transformation of Neisseria gonorrhoeae: from DNA donation to homologous recombination

HL Hamilton, JP Dillard - Molecular microbiology, 2006 - Wiley Online Library
Gonococci undergo frequent and efficient natural transformation. Transformation occurs so
often that the population structure is panmictic, with only one long‐lived clone having been …

Restriction of plasmid DNA during transformation but not conjugation in Neisseria gonorrhoeae

DC Stein, S Gregoire, A Piekarowicz - Infection and immunity, 1988 - Am Soc Microbiol
Neisseria gonorrhoeae strains WR302 and PGH3-2 were characterized with respect to their
restriction-modification phenotype. WR302 DNA was cleaved by HaeIII, indicating the lack of …

New functional identity for the DNA uptake sequence in transformation and its presence in transcriptional terminators

OH Ambur, SA Frye, T Tønjum - Journal of bacteriology, 2007 - Am Soc Microbiol
The frequently occurring DNA uptake sequence (DUS), recognized as a 10-bp repeat, is
required for efficient genetic transformation in the human pathogens Neisseria meningitidis …

[HTML][HTML] Multisite transformation in Neisseria gonorrhoeae: insights on transformations mechanisms and new genetic modification protocols

VY Seow, O Tsygelnytska, N Biais - Frontiers in Microbiology, 2023 - frontiersin.org
Natural transformation, or the uptake of naked DNA from the external milieu by bacteria,
holds a unique place in the history of biology. This is both the beginning of the realization of …

Uptake-sequence-independent DNA transformation exists in Neisseria gonorrhoeae

S Boyle-Vavra, HS Seifert - Microbiology, 1996 - microbiologyresearch.org
A DNA transformation dose-response curve of piliated (P+) gonococci with the use of cloned
DNA containing a pilE2-cat fusion showed saturation at high and low levels of transforming …