Comparative study on density gradients and swim‐up preparation techniques utilizing neat and cryopreserved spermatozoa

SSR Allamaneni, A Agarwal, S Rama… - Asian journal of …, 2005 - Wiley Online Library
Abstract Aim: To 1) compare post‐wash and post‐thaw parameters of sperm processed with
PureSperm density gradient technique and swim‐up method; and 2) test the efficacy of two …

Comparison of motility and flow cytometric assessments of seminal quality in fresh, 24‐hour extended and cryopreserved human spermatozoa

RY Kramer, DL Garner, ES Bruns… - Journal of …, 1993 - Wiley Online Library
Functional differences among fresh 24‐hour extended and cryopreserved human
spermatozoa were assessed using both computer‐assisted semen analysis (CASA) and …

Spermatozoal characteristics from fresh and frozen donor semen and their correlation with fertility outcome after intrauterine insemination

PB Marshburn, D McIntire, BR Carr, W Byrd - Fertility and sterility, 1992 - Elsevier
Objective To determine if conventional sperm parameters, specific characteristics of sperm
motion determined by computer-aided semen analysis (CASA), sperm penetration assay …

Concentrating human sperm before cryopreservation

T Kobayashi, S Kaneko, I Hara, YJ Park, H Sato… - Andrologia, 1991 - Wiley Online Library
To improve the quality of cryo‐preserved sperm, ejaculated semen was concentrated prior to
freezing by means of continuous‐step density gradient centrifugation. Freezing was …

[HTML][HTML] Cryopreservation of human semen and prepared sperm: effects on motility parameters and DNA integrity

ET Donnelly, N McClure, SEM Lewis - Fertility and sterility, 2001 - Elsevier
Objective: To investigate effects of cryopreservation on sperm motility and DNA integrity.
Design: Pre-cryopreservation and post-cryopreservation analysis of motility and DNA …

[HTML][HTML] Rapid freezing versus slow programmable freezing of human spermatozoa

T Vutyavanich, W Piromlertamorn, S Nunta - Fertility and sterility, 2010 - Elsevier
OBJECTIVE: To compare the efficacy of rapid freezing (RF) and slow programmable
freezing (SPF) of human spermatozoa. DESIGN: Experimental study. SETTING: University …

A Mono-Percoll separation technique improves sperm recovery of normal and male factor specimens when compared with the swim-up technique

S Shalika, K Dugan, D Pelesh, S Padilla - Human Reproduction, 1995 - academic.oup.com
The purpose of this study was to determine the effects of a simplified 80% Mono-Percoll
sperm separation procedure on both normal and male factor semen samples compared with …

Is pre-freeze sperm preparation more advantageous than post-freeze?

DA Androni, S Dodds, M Tomlinson… - Reproduction and …, 2021 - raf.bioscientifica.com
Human sperm cryopreservation is characterised to this day by sub-optimal success rates.
Interestingly, a traditional approach to improving post-thaw outcome has been to integrate …

[HTML][HTML] Post-thaw recovery of rare or very low concentrations of cryopreserved human sperm

M Kathrins, N Abhyankar, O Shoshany, J Liebermann… - Fertility and sterility, 2017 - Elsevier
Objective To analyze cases in which no sperm could be identified after thawing among
cryopreserved samples of rare or very low concentrations of sperm. Design Retrospective …

Comparison of six density gradient media for selection of cryopreserved donor spermatozoa

N Mousset‐Siméon, N Rives, L Masse… - Journal of …, 2004 - Wiley Online Library
The aim of our study was to evaluate the efficiency of 4 density gradient media for motile
cryopreserved spermatozoa selection to Percoll (Kabi Pharmacia, Uppsala, Sweden) and to …