Recently developed CRISPR-mediated base editors, which enable the generation of
numerous nucleotide changes in target genomic regions, have been widely adopted for …

The CRISPR/Cas system has rapidly become the preferred tool for genome engineering in
various organisms due to high efficiency, specificity, simplicity and versatility. Currently …

CRISPR technologies enabling precise genome manipulation are valuable for gene function
studies and molecular crop breeding. However, the requirement of a protospacer adjacent …

Gene-targeting technologies using sequence specific nucleases have been widely adopted
to induce genetic modifications in both plant molecular biology research and crop …

The Cas9/sgRNA of the CRISPR/Cas system has emerged as a robust technology for
targeted gene editing in various organisms, including plants, where Cas9/sgRNA-mediated …

Precise and straightforward methods to edit the plant genome are much needed for
functional genomics and crop improvement. Recently, RNA-guided genome editing using …

Base editing is a novel genome editing strategy that enables irreversible base conversion at
target loci without the need for double stranded break induction or homology‐directed …

The CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR
associated protein 9) system is revolutionizing genome editing due to its high efficiency, low …

Planted to two million ha farmland and yielding more than 70 million tons per year,
watermelon dominates the fruit market in China. However, with a low planting density and …

PAM‐relaxed Cas9 nucleases, cytosine base editors and adenine base editors are
promising tools for precise genome editing in plants. However, their genome‐wide off‐target …