Protein inhibitors of CRISPR-Cas9

J Bondy-Denomy - ACS chemical biology, 2018 - ACS Publications
Bacteria are under constant predation from viruses, called bacteriophages (phages). This
threat has driven the evolution of multiple defense systems, including the CRISPR-Cas …

The basic building blocks and evolution of CRISPR–Cas systems

KS Makarova, YI Wolf, EV Koonin - Biochemical Society …, 2013 - portlandpress.com
CRISPR (clustered regularly interspaced short palindromic repeats)–Cas (CRISPR-
associated) is an adaptive immunity system in bacteria and archaea that functions via a …

The Card1 nuclease provides defence during type III CRISPR immunity

JT Rostøl, W Xie, V Kuryavyi, P Maguin, K Kao… - Nature, 2021 - nature.com
In the type III CRISPR–Cas immune response of prokaryotes, infection triggers the
production of cyclic oligoadenylates that bind and activate proteins that contain a CARF …

[HTML][HTML] CRISPR–Cas: complex functional networks and multiple roles beyond adaptive immunity

G Faure, KS Makarova, EV Koonin - Journal of molecular biology, 2019 - Elsevier
CRISPR–Cas is a prokaryotic adaptive immune system that functions by incorporating
fragments of foreign DNA into CRISPR arrays. The arrays containing spacers derived from …

[HTML][HTML] Structural rearrangements allow nucleic acid discrimination by type ID Cascade

EA Schwartz, TM McBride, JPK Bravo, D Wrapp… - Nature …, 2022 - nature.com
CRISPR-Cas systems are adaptive immune systems that protect prokaryotes from foreign
nucleic acids, such as bacteriophages. Two of the most prevalent CRISPR-Cas systems …

Characterization and repurposing of type I and type II CRISPR–Cas systems in bacteria

C Hidalgo-Cantabrana, YJ Goh, R Barrangou - Journal of molecular biology, 2019 - Elsevier
CRISPR–Cas systems constitute the adaptive immune system of bacteria and archaea, as a
sequence-specific nucleic acid targeting defense mechanism. The sequence-specific …

[PDF][PDF] Structure and mechanism of a cyclic trinucleotide-activated bacterial endonuclease mediating bacteriophage immunity

RK Lau, Q Ye, EA Birkholz, KR Berg, L Patel… - Molecular cell, 2020 - cell.com
Bacteria possess an array of defenses against foreign invaders, including a broadly
distributed bacteriophage defense system termed CBASS (cyclic oligonucleotide-based anti …

[PDF][PDF] DNA unwinding is the primary determinant of CRISPR-Cas9 activity

S Gong, HH Yu, KA Johnson, DW Taylor - Cell reports, 2018 - cell.com
Bacterial adaptive immunity utilizes RNA-guided surveillance complexes comprising Cas
proteins together with CRISPR RNAs (crRNAs) to target foreign nucleic acids for destruction …

[PDF][PDF] Cas5d protein processes pre-crRNA and assembles into a cascade-like interference complex in subtype IC/Dvulg CRISPR-Cas system

KH Nam, C Haitjema, X Liu, F Ding, H Wang… - Structure, 2012 - cell.com
Clustered regularly interspaced short palindromic repeats (CRISPRs), together with an
operon of CRISPR-associated (Cas) proteins, form an RNA-based prokaryotic immune …

Three CRISPR-Cas immune effector complexes coexist in Pyrococcus furiosus

S Majumdar, P Zhao, NT Pfister, M Compton, S Olson… - Rna, 2015 - rnajournal.cshlp.org
CRISPR-Cas immune systems function to defend prokaryotes against potentially harmful
mobile genetic elements including viruses and plasmids. The multiple CRISPR-Cas systems …