Transposition activity in bacteria is generally maintained at a low level. The activity of mobile
DNA elements can be controlled by bacterially encoded global regulators. Regulation of …

Transpositional activity of mobile elements is not constant. Conditional regulation of host
factors involved in transposition may severely change the activity of mobile elements. We …

We have previously shown that both ends of the Tn 3 family transposon Tn 4652 contain
integration host factor (IHF) binding sites and that IHF positively regulates expression of the …

Transposition is a DNA reorganization reaction potentially deleterious for the host. The
frequency of transposition is limited by the amount of transposase. Therefore, strict …

Tn 4652 is a derivative of the toluene degradation transposon Tn 4651 that belongs to the
Tn 3 family of transposons (M. Tsuda and T. Iino, Mol. Gen. Genet. 210: 270–276, 1987). We …

Here, a protocol is outlined to perform live, real-time imaging of transposable element
activity in live bacterial cells using a suite of fluorescent reporters coupled to transposition. In …

We analyzed the target preferences of a Tn 3 family transposon Tn 4652. Alignment of 93
different insertion sites revealed a consensus sequence which resembles that of Tn 3 …

Conjugative transposition of transposon Tn916 has been shown to proceed by excision of
the transposon in the donor strain and insertion of this element in the recipient. This process …

We show in this work that disruption of the hfq gene in Escherichia coli causes a large
increase in IS10 transposition when IS10 is present on a multi‐copy plasmid. Hfq is an RNA …