Real-time Analysis of Metabolic Activity Within Lactobacillus acidophilus by Phasor Fluorescence Lifetime Imaging Microscopy of NADH

K Torno, BK Wright, MR Jones, MA Digman… - Current …, 2013 - Springer
Nicotinamide adenine dinucleotide (NADH) is an endogenous fluorescent molecule
commonly used as a metabolic biomarker. Fluorescence lifetime imaging microscopy (FLIM) …

Metabolic fingerprinting of bacteria by fluorescence lifetime imaging microscopy

A Bhattacharjee, R Datta, E Gratton, AI Hochbaum - Scientific reports, 2017 - nature.com
Bacterial populations exhibit a range of metabolic states influenced by their environment,
intra-and interspecies interactions. The identification of bacterial metabolic states and …

Metabolism of HeLa cells revealed through autofluorescence lifetime upon infection with enterohemorrhagic Escherichia coli

TY Buryakina, PT Su, WJ Syu… - Journal of …, 2012 - spiedigitallibrary.org
Fluorescence lifetime imaging microscopy (FLIM) is a sensitive technique in monitoring
functional and conformational states of nicotinamide adenine dinucleotide reduced (NADH) …

Enhanced quantification of metabolic activity for individual adipocytes by label-free FLIM

M Evers, N Salma, S Osseiran, M Casper… - Scientific Reports, 2018 - nature.com
Fluorescence lifetime imaging microscopy (FLIM) of intrinsic fluorophores such as
nicotinamide adenine dinucleotide (NADH) allows for label-free quantification of metabolic …

Longitudinal monitoring of cell metabolism in biopharmaceutical production using label‐free fluorescence lifetime imaging microscopy

SM Sternisha, P Mukherjee, A Alex… - Biotechnology …, 2021 - Wiley Online Library
Chinese hamster ovary (CHO) cells are routinely used in the biopharmaceutical industry for
production of therapeutic monoclonal antibodies (mAbs). Although multiple offline and time …

Determination of the metabolic index using the fluorescence lifetime of free and bound nicotinamide adenine dinucleotide using the phasor approach

S Ranjit, L Malacrida, M Stakic… - Journal of …, 2019 - Wiley Online Library
The fluorescence lifetime of nicotinamide adenine dinucleotide (NADH) is commonly used in
conjunction with the phasor approach as a molecular biomarker to provide information on …

An automated multiwell plate reading FLIM microscope for live cell autofluorescence lifetime assays

DJ Kelly, SC Warren, S Kumar, JL Lagarto… - Journal of Innovative …, 2014 - World Scientific
Fluorescence lifetime imaging (FLIM) is increasingly used to read out cellular
autofluorescence originating from the coenzyme NADH in the context of investigating cell …

Design of a line-of-sight fluorescence-based imaging diagnostic for classification of microbe species

JM Herzog, V Sick - Measurement Science and Technology, 2023 - iopscience.iop.org
Fluorescence imaging of certain biochemicals, including flavins and pyridine nucleotides,
has utility in characterizing the metabolic state of tissue and in discriminating between …

NAD (P) H fluorescence lifetime measurements in fixed biological tissues

JV Chacko, KW Eliceiri - Methods and Applications in …, 2019 - iopscience.iop.org
Autofluorescence based fluorescence lifetime imaging microscopy (AF-FLIM) techniques
have come a long way from early studies on cancer characterization and have now been …

[HTML][HTML] Quantification of the metabolic state in cell-model of Parkinson's disease by fluorescence lifetime imaging microscopy

S Chakraborty, FS Nian, JW Tsai, A Karmenyan… - Scientific reports, 2016 - nature.com
Intracellular endogenous fluorescent co-enzymes, reduced nicotinamide adenine
dinucleotide (NADH) and flavin adenine dinucleotide (FAD), play a pivotal role in cellular …