Competitive inhibition ELISA for quantification of Ara h 1 and Ara h 2, the major allergens of peanuts

DA Schmitt, H Cheng, SJ Maleki… - Journal of AOAC …, 2004 - academic.oup.com
Allergies to peanuts are becoming an increasingly important health problem as a result of
the persistence and severity of the reaction in allergic individuals. Because no treatment …

Development of sandwich ELISA and immunochromatographic strip for the detection of peanut allergen Ara h 2

J Peng, S Song, L Liu, H Kuang, C Xu - Food Analytical Methods, 2015 - Springer
In order to develop highly sensitive immunoassays for detecting the major peanut allergen,
Ara h 2, we prepared six monoclonal antibodies (mAb) with high affinity to Ara h 2. Using …

Monitoring peanut allergen in food products by measuring Ara h 1

A Pomés, RM Helm, GA Bannon, AW Burks… - Journal of Allergy and …, 2003 - Elsevier
Background: Peanut allergy is an important health problem in the United States, affecting
approximately 0.6% of children. Inadvertent exposure to peanut is a risk factor for life …

Ara h 6 complements Ara h 2 as an important marker for IgE reactivity to peanut

AE Koid, MD Chapman, RG Hamilton… - Journal of agricultural …, 2014 - ACS Publications
The similarities of two major peanut allergens, Ara h 2 and Ara h 6, in molecular size, amino
acid sequence, and structure have made it difficult to obtain natural Ara h 6 free of Ara h 2 …

[HTML][HTML] Development of a monoclonal antibody-based sandwich ELISA for peanut allergen Ara h 1 in food

J Peng, S Song, L Xu, W Ma, L Liu, H Kuang… - International Journal of …, 2013 - mdpi.com
We have established a highly sensitive sandwich enzyme-linked immunosorbent assay
(ELISA) based on two monoclonal antibodies (mAb) to measure the content of the major …

Quantification of Ara h 1 in peanuts: why roasting makes a difference

A Pomés, CL Butts… - Clinical & Experimental …, 2006 - Wiley Online Library
Background Increased allergenicity of roasted vs. raw peanut has been reported by showing
higher IgE binding to roasted peanut extracts. Objective To study the effect of roasting on Ara …

Can commercial peanut assay kits detect peanut allergens?

MCL Nogueira, R McDonald, C Westphal… - Journal of AOAC …, 2004 - academic.oup.com
Peanut is the food group mostly associated with severe and fatal allergic reactions. In the
United States, more than 90% of peanut-allergic individuals' serum IgE recognized peanut …

Peanut varieties with reduced Ara h 1 content indicating no reduced allergenicity

S Krause, T Latendorf, H Schmidt… - Molecular nutrition & …, 2010 - Wiley Online Library
Peanut allergy is a major cause of food‐induced severe anaphylactic reactions. To date, no
medical care is available to prevent and treat peanut allergy and therefore hypoallergenic …

Relevance of Ara h1, Ara h2 and Ara h3 in peanut‐allergic patients, as determined by immunoglobulin E Western blotting, basophil–histamine release and …

SJ Koppelman, M Wensing, M Ertmann… - Clinical & …, 2004 - Wiley Online Library
Background A number of allergenic proteins in peanut has been described and the relative
importance of these allergens is yet to be determined. Objectives We have investigated the …

Quantification of major peanut allergens Ara h 1 and Ara h 2 in the peanut varieties Runner, Spanish, Virginia, and Valencia, bred in different parts of the world

SJ Koppelman, RAA Vlooswijk, LMJ Knippels… - Allergy, 2001 - Wiley Online Library
Background: The serology of peanut allergy seems to be different in various parts of the
world. We analyzed the composition of 13 samples of three varieties of peanut in order to …