We generated by gene disruption Dictyostelium cells that lacked both the F-actin cross-
linking proteins, a-actinin and gelation factor. Several major cell functions, such as growth …

Actin-binding proteins are known to regulate in vitro the assembly of actin into
supramolecular structures, but evidence for their activities in living nonmuscle cells is …

The actin cytoskeleton is implicated in many cellular processes, such as cell adhesion,
locomotion, contraction and cytokinesis, which are central to any development. The extent of …

We have studied the effect of the Dictyostelium discoideum 30,000-D actin-bundling protein
on the assembly and disassembly of pyrenyl-labeled actin in vitro. The results indicate that …

Cells lacking the Dictyostelium 34,000-D actin-bundling protein, a calcium-regulated actin
cross-linking protein, were created to probe the function of this polypeptide in living cells …

The contribution of three actin cross-linking proteins, α-actinin (αA), gelation factor (ABP-
120), and the 34 kDa actin-bundling protein to cellular functions has been studied in three …

We generated Dictyostelium double mutants lacking the two F-actin crosslinking proteins α-
actinin and gelation factor by inactivating the corresponding genes via homologous …

A clone coding for the F-actin cross-linking protein alpha-actinin was obtained by screening
a genomic library of Dictyostelium discoideum DNA in lambda gt11 with monoclonal …

The actin cytoskeleton is sensitive to changes in calcium, which affect contractility, actin-
severing proteins, actin-crosslinking proteins and calmodulin-regulated enzymes. To dissect …

A monoclonal antibody, mAb 47‐19‐2, was used to study the subunit topology of the rod‐
shaped alpha‐actinin molecules of Dictyostelium discoideum and to screen for mutants …