Improving the cytoplasmic maturation of bovine oocytes matured in vitro with intracellular and/or extracellular antioxidants is not associated with increased rates of …

NAS Rocha-Frigoni, BCS Leão, PC Dall'Acqua… - Theriogenology, 2016 - Elsevier
The production of reactive oxygen species (ROS) is a normal process that occurs in the
cellular mitochondrial respiratory chain. However, an increase in ROS levels during in vitro …

[HTML][HTML] Current advances in bovine in Vitro maturation and embryo production using different antioxidants: a review

R Naspinska, MH Moreira da Silva… - Journal of …, 2023 - mdpi.com
In vitro maturation (IVM) is one of the most important steps in in vitro embryo production
(IVEP). It is a complicated procedure in which nuclear and cytoplasmatic changes in oocytes …

Effects of gaseous atmosphere and antioxidants on the development and cryotolerance of bovine embryos at different periods of in vitro culture

NA de Souza Rocha-Frigoni, BC da Silva Leão… - Zygote, 2015 - cambridge.org
This study examined the effects of antioxidant supplementation and O2 tension on embryo
development, cryotolerance and intracellular reactive oxygen species (ROS) levels. The …

Synergistic effects of glutathione and β-mercaptoethanol treatment during in vitro maturation of porcine oocytes on early embryonic development in a culture system …

C Choe, YW Shin, EJ Kim, SR Cho, HJ Kim… - … of Reproduction and …, 2010 - jstage.jst.go.jp
Various methods have been used to remove reactive oxygen species (ROS) generated from
in vitro culture (IVC) conditions that can cause cell injury or death, including the application …

Effects of supplementation of medium with different antioxidants during in vitro maturation of bovine oocytes on subsequent embryo production

TC Sovernigo, PR Adona, PS Monzani… - … in Domestic Animals, 2017 - Wiley Online Library
Contents The aim of this study was to assess the effects of different antioxidants on the
levels of reactive oxygen species (ROS) and glutathione (GSH) in oocytes during in vitro …

Antioxidant requirements for bovine oocytes varies during in vitro maturation, fertilization and development

AA Ali, JF Bilodeau, MA Sirard - Theriogenology, 2003 - Elsevier
Antioxidants may be beneficial additives to synthetic culture media because these well
defined media lack serum or other macromolecules that serve as reactive oxygen species …

Recovery of mitochondrial function and endogenous antioxidant systems in vitrified bovine oocytes during extended in vitro culture

XM Zhao, WH Du, D Wang, HS Hao… - Molecular …, 2011 - Wiley Online Library
This study was designed to examine the recovery of mitochondrial function and endogenous
antioxidant systems in vitrified oocytes during extended incubations. After 16 hr of in vitro …

Mitochondrial activity during pre-maturational culture in in vitro-grown bovine oocytes is related to maturational and developmental competences

W Huang, SS Kang, K Nagai… - Reproduction …, 2016 - CSIRO Publishing
The objective of this study was to investigate the dynamics of mitochondrial activity in in vitro-
grown (IVG) bovine oocytes during pre-maturational culture (pre-IVM) and its relationship to …

Reduced levels of intracellular reactive oxygen species and apoptotic status are not correlated with increases in cryotolerance of bovine embryos produced in vitro in …

NAS Rocha-Frigoni, BCS Leão… - Reproduction …, 2014 - CSIRO Publishing
The effects of intracellular (cysteine and β-mercaptoethanol) and extracellular (catalase)
antioxidant supplementation at different times during in vitro production (IVM and/or in vitro …

Transporting bovine oocytes in a medium supplemented with different macromolecules and antioxidants: Effects on nuclear and cytoplasmic maturation and …

M Ambrogi, PC Dall'Acqua… - … in Domestic Animals, 2017 - Wiley Online Library
Contents We investigated whether supplementing the medium used to transport bovine
oocytes with different macromolecules [foetal calf serum (FCS) or bovine serum albumin …