Fast fluorescence lifetime imaging techniques: A review on challenge and development

X Liu, D Lin, W Becker, J Niu, B Yu, L Liu… - Journal of Innovative …, 2019 - World Scientific
Fluorescence lifetime imaging microscopy (FLIM) is increasingly used in biomedicine,
material science, chemistry, and other related research fields, because of its advantages of …

Recent innovations in fluorescence lifetime imaging microscopy for biology and medicine

R Datta, A Gillette, M Stefely… - Journal of biomedical …, 2021 - spiedigitallibrary.org
Significance: Fluorescence lifetime imaging microscopy (FLIM) measures the decay rate of
fluorophores, thus providing insights into molecular interactions. FLIM is a powerful …

High-speed multispectral fluorescence lifetime imaging implementation for in vivo applications

S Shrestha, BE Applegate, J Park, X Xiao, P Pande… - Optics Letters, 2010 - opg.optica.org
Fluorescence lifetime imaging microscopy (FLIM) offers a noninvasive approach for
characterizing the biochemical composition of biological tissue. In recent years, there has …

Fluorescence lifetime imaging (FLIM): Basic concepts and some recent developments

K Suhling, LM Hirvonen, JA Levitt, PH Chung… - Medical photonics, 2015 - Elsevier
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the
environment and interaction of fluorescent probes. It can report on photophysical events that …

Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications

R Datta, TM Heaster, JT Sharick… - … of biomedical optics, 2020 - spiedigitallibrary.org
Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …

FLIM as a promising tool for cancer diagnosis and treatment monitoring

Y Ouyang, Y Liu, ZM Wang, Z Liu, M Wu - Nano-Micro Letters, 2021 - Springer
Fluorescence lifetime imaging microscopy (FLIM) has been rapidly developed over the past
30 years and widely applied in biomedical engineering. Recent progress in fluorophore …

Time-domain fluorescence lifetime imaging applied to biological tissue

D Elson, J Requejo-Isidro, I Munro, F Reavell… - Photochemical & …, 2004 - Springer
Fluorescence lifetime imaging (FLIM) is a functional imaging methodology that can provide
information, not only concerning the localisation of specific fluorophores, but also about the …

A novel fluorescence lifetime imaging system that optimizes photon efficiency

RA Colyer, C Lee, E Gratton - Microscopy research and …, 2008 - Wiley Online Library
Fluorescence lifetime imaging (FLIM) is a powerful microscopy technique for providing
contrast of biological and other systems by differences in molecular species or their …

Fluorescence lifetime imaging–techniques and applications

W Becker - Journal of microscopy, 2012 - Wiley Online Library
Fluorescence lifetime imaging (FLIM) uses the fact that the fluorescence lifetime of a
fluorophore depends on its molecular environment but not on its concentration. Molecular …

Mesoscopic fluorescence lifetime imaging: Fundamental principles, clinical applications and future directions

A Alfonso‐Garcia, J Bec, B Weyers… - Journal of …, 2021 - Wiley Online Library
Fluorescence lifetime imaging (FLIm) is an optical spectroscopic imaging technique capable
of real‐time assessments of tissue properties in clinical settings. Label‐free FLIm is sensitive …