A modified phasor approach for analyzing time‐gated fluorescence lifetime images

F Fereidouni, A Esposito, GA Blab… - Journal of …, 2011 - Wiley Online Library
Fluorescence lifetime imaging is a versatile tool that permits mapping the biochemical
environment in the cell. Among various fluorescence lifetime imaging techniques, time …

Artificial neural network approaches for fluorescence lifetime imaging techniques

G Wu, T Nowotny, Y Zhang, HQ Yu, DDU Li - Optics letters, 2016 - opg.optica.org
A novel high-speed fluorescence lifetime imaging (FLIM) analysis method based on artificial
neural networks (ANN) has been proposed. In terms of image generation, the proposed …

Fluorescence lifetime-resolved imaging

YC Chen, RM Clegg - Photosynthesis research, 2009 - Springer
This is a short account of fluorescence lifetime-resolved imaging, in order to acquaint
readers who are not experts with the basic methods for measuring lifetime-resolved signals …

Photon budget analysis for fluorescence lifetime imaging microscopy

Q Zhao, IT Young, JGS De Jong - Journal of Biomedical Optics, 2011 - spiedigitallibrary.org
We have constructed a mathematical model to analyze the photon efficiency of frequency-
domain fluorescence lifetime imaging microscopy (FLIM). The power of the light source …

Fit-free analysis of fluorescence lifetime imaging data using the phasor approach

S Ranjit, L Malacrida, DM Jameson, E Gratton - Nature protocols, 2018 - nature.com
Fluorescence lifetime imaging microscopy (FLIM) is used in diverse disciplines, including
biology, chemistry and biophysics, but its use has been limited by the complexity of the data …

[HTML][HTML] Rapid global fitting of large fluorescence lifetime imaging microscopy datasets

SC Warren, A Margineanu, D Alibhai, DJ Kelly… - PloS one, 2013 - journals.plos.org
Fluorescence lifetime imaging (FLIM) is widely applied to obtain quantitative information
from fluorescence signals, particularly using Förster Resonant Energy Transfer (FRET) …

Fluorescence lifetime imaging microscopy: Pixel‐by‐pixel analysis of phase‐modulation data

TWJ Gadella Jr, RM Clegg, TM Jovin - Bioimaging, 1994 - Wiley Online Library
The novel techniques of fluorescence lifetime imaging (FLI) and FLI‐microscopy (FLIM) in
the frequency domain enable the mapping of the spatial distribution of fluorescence lifetimes …

[HTML][HTML] Global analysis of fluorescence lifetime imaging microscopy data

PJ Verveer, A Squire, PIH Bastiaens - Biophysical journal, 2000 - cell.com
Global analysis techniques are described for frequency domain fluorescence lifetime
imaging microscopy (FLIM) data. These algorithms exploit the prior knowledge that only a …

[HTML][HTML] Robust Bayesian fluorescence lifetime estimation, decay model selection and instrument response determination for low-intensity FLIM imaging

MI Rowley, ACC Coolen, B Vojnovic, PR Barber - PLoS one, 2016 - journals.plos.org
We present novel Bayesian methods for the analysis of exponential decay data that exploit
the evidence carried by every detected decay event and enables robust extension to …

[HTML][HTML] Fast timing techniques in FLIM applications

LM Hirvonen, K Suhling - Frontiers in Physics, 2020 - frontiersin.org
Fluorescence lifetime imaging (FLIM) is increasingly used in many scientific disciplines,
including biological and medical research, materials science and chemistry. The …