Label-free chemical imaging of fungal spore walls by Raman microscopy and multivariate curve resolution analysis

H Noothalapati, T Sasaki, T Kaino, M Kawamukai… - Scientific reports, 2016 - nature.com
Fungal cell walls are medically important since they represent a drug target site for
antifungal medication. So far there is no method to directly visualize structurally similar cell …

Principal component analysis facilitated fast and noninvasive Raman spectroscopic imaging of plant cell wall pectin distribution and interaction with enzymatic …

Q He, OA Zabotina, C Yu - Journal of Raman Spectroscopy, 2020 - Wiley Online Library
The plant cell wall is a complex network of polysaccharides. A better understanding of the
plant cell wall polysaccharide content, distribution, and interactions among them could be …

Inhomogeneous molecular distributions and cytochrome types and redox states in fungal cells revealed by Raman hyperspectral imaging using multivariate curve …

M Yasuda, N Takeshita, S Shigeto - Analytical chemistry, 2019 - ACS Publications
Hyphae of filamentous fungi consist of compartments that are distinct both spatially and
functionally, thereby forming a unique multicellular system. Much work has been done …

Proof-of-principle for SERS imaging of Aspergillus nidulans hyphae using in vivo synthesis of gold nanoparticles

MA Prusinkiewicz, F Farazkhorasani, JJ Dynes, J Wang… - Analyst, 2012 - pubs.rsc.org
High spatial resolution methods to assess the physiology of growing cells should permit
analysis of fungal biochemical composition. Whole colony methods cannot capture the …

Molecular architecture of fungal cell walls revealed by solid-state NMR

X Kang, A Kirui, A Muszyński, MCD Widanage… - Nature …, 2018 - nature.com
The high mortality of invasive fungal infections, and the limited number and inefficacy of
antifungals necessitate the development of new agents with novel mechanisms and targets …

Solid-state NMR molecular snapshots of Aspergillus fumigatus cell wall architecture during a conidial morphotype transition

G Lamon, A Lends, I Valsecchi… - Proceedings of the …, 2023 - National Acad Sciences
While establishing an invasive infection, the dormant conidia of Aspergillus fumigatus transit
through swollen and germinating stages, to form hyphae. During this morphotype transition …

Deuterium-labeled Raman tracking of glucose accumulation and protein metabolic dynamics in Aspergillus nidulans hyphal tips

M Yasuda, N Takeshita, S Shigeto - Scientific reports, 2021 - nature.com
Filamentous fungi grow exclusively at their tips, where many growth-related fungal
processes, such as enzyme secretion and invasion into host cells, take place. Hyphal tips …

Enhanced method for high spatial resolution surface imaging and analysis of fungal spores using scanning electron microscopy

G Venkatesh Babu, P Perumal, S Muthu, S Pichai… - Scientific Reports, 2018 - nature.com
Efficient, fast and new micro-analytical methods for characterization of ultrastructures of
fungal spores with electron microscopy are very much required and essential. SEM analysis …

Solid-state NMR analysis of unlabeled fungal cell walls from Aspergillus and Candida species

LD Fernando, MCD Widanage, SC Shekar… - Journal of Structural …, 2022 - Elsevier
Fungal infections cause high mortality in immunocompromised individuals, which has
emerged as a significant threat to human health. The efforts devoted to the development of …

Stimulated Raman Imaging Reveals Aberrant Lipogenesis as a Metabolic Marker for Azole-Resistant Candida albicans

CW Karanja, W Hong, W Younis… - Analytical …, 2017 - ACS Publications
Candida albicans is the single most prevalent cause of fungal bloodstream infections
worldwide causing significant mortality as high as 50 percent. This high mortality rate is, in …