Development and validation of InnoQuant® HY, a system for quantitation and quality assessment of total human and male DNA using high copy targets

A Loftus, G Murphy, H Brown, A Montgomery… - Forensic Science …, 2017 - Elsevier
The development and validation of InnoQuant® HY, a real-time PCR system containing four
DNA targets—two RE autosomal targets of different sizes, male specific targets, and an …

[HTML][HTML] Human DNA quantification and sample quality assessment: Developmental validation of the PowerQuantĘr) system

MM Ewing, JM Thompson, RS McLaren… - Forensic Science …, 2016 - Elsevier
Quantification of the total amount of human DNA isolated from a forensic evidence item is
crucial for DNA normalization prior to short tandem repeat (STR) DNA analysis and a federal …

Developmental Validation of the Quantifiler® Duo DNA Quantification Kit for Simultaneous Quantification of Total Human and Human Male DNA and Detection of …

M Barbisin, R Fang, CE O'Shea… - Journal of forensic …, 2009 - Wiley Online Library
The Quantifiler® Duo DNA Quantification kit enables simultaneous quantification of human
DNA and human male DNA as well as detection of inhibitors of PCR in a single real‐time …

A performance study on three qPCR quantification kits and their compatibilities with the 6-dye DNA profiling systems

S Lin, C Li, SCY Ip - Forensic Science International: Genetics, 2018 - Elsevier
DNA quantification plays an integral role in forensic DNA profiling. Not only does it estimate
the total amount of amplifiable human autosomal and male DNA to ensure optimal …

Assessing DNA Degradation through Differential Amplification Efficiency of Total Human and Human Male DNA in a Forensic qPCR Assay

E Chierto, S Aneli, N Nocco, A Riem, M Onofri… - Genes, 2024 - mdpi.com
The assessment of degradation is crucial for the analysis of human DNA samples isolated
from forensic specimens. Forensic quantitative PCR (qPCR) assays can include multiple …

Development and validation of InnoQuant™, a sensitive human DNA quantitation and degradation assessment method for forensic samples using high copy number …

GM Pineda, AH Montgomery, R Thompson… - Forensic Science …, 2014 - Elsevier
There is a constant need in forensic casework laboratories for an improved way to increase
the first-pass success rate of forensic samples. The recent advances in mini STR analysis …

Development and usage of a NIST standard reference material for real time PCR quantitation of human DNA

PM Vallone, MC Kline, DL Duewer, AE Decker… - Forensic Science …, 2008 - Elsevier
National Institute of Standards and Technology SRM 2372 human DNA quantitation
standard has been produced to support the need for a human-specific DNA quantitation …

[HTML][HTML] Genomic Multicopy Loci Targeted by Current Forensic Quantitative PCR Assays

R Jäger - Genes, 2024 - mdpi.com
Modern forensic DNA quantitation assays provide information on the suitability of a DNA
extract for a particular type of analysis, on the amount of sample to put into the analysis in …

Development and validation of the Investigator® Quantiplex Pro Kit for qPCR-based examination of the quantity and quality of human DNA in forensic samples

M Vraneš, M Scherer, K Elliott - Forensic Science International: Genetics …, 2017 - Elsevier
We developed and validated the Investigator Quantiplex Pro Kit which quantifies human and
male DNA in a sample using quantitative real-time PCR. An internal PCR control (IPC) is …

Developmental validation of the Quantifiler® HP and Trio Kits for human DNA quantification in forensic samples

A Holt, SC Wootton, JJ Mulero, PM Brzoska… - Forensic Science …, 2016 - Elsevier
The quantification of human genomic DNA is a necessary first step in the DNA casework
sample analysis workflow. DNA quantification determines optimal sample input amounts for …