MiniUIB, a novel minitransposon-based system for stable insertion of foreign DNA into the genomes of gram-negative and gram-positive bacteria
JA Christie-Oleza, I Brunet-Galmés… - Applied and …, 2013 - Am Soc Microbiol
Transposition of the insertion sequence (IS) IS Ppu12 is actively induced after conjugative
interaction. The transposase of this IS can act in trans on structures flanked by inverted …
interaction. The transposase of this IS can act in trans on structures flanked by inverted …
Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in gram-negative eubacteria
V De Lorenzo, M Herrero, U Jakubzik… - Journal of …, 1990 - Am Soc Microbiol
A collection of Tn5-derived minitransposons has been constructed that simplifies
substantially the generation of insertion mutants, in vivo fusions with reporter genes, and the …
substantially the generation of insertion mutants, in vivo fusions with reporter genes, and the …
Random transposon vectors pUTTns for the markerless integration of exogenous genes into gram-negative eubacteria chromosomes
R Li, G Wang, B Shen, R Wang, Y Song, S Li… - Journal of microbiological …, 2009 - Elsevier
A set of random transposon vectors pUTTns that facilitates the markerless integration of new
functions into the chromosome of gram-negative bacteria has been developed. The vectors …
functions into the chromosome of gram-negative bacteria has been developed. The vectors …
Characterization of the Pseudomonas putida Mobile Genetic Element ISPpu10: an Occupant of Repetitive Extragenic Palindromic Sequences
MI Ramos-González, MJ Campos, JL Ramos… - Journal of …, 2006 - Am Soc Microbiol
We have characterized the Pseudomonas putida KT2440 insertion element ISPpu 10. This
insertion sequence encodes a transposase which exhibits homology to the transposases …
insertion sequence encodes a transposase which exhibits homology to the transposases …
Mini-Tn10 transposon derivatives for insertion mutagenesis and gene delivery into the chromosome of gram-negative bacteria
MF Alexeyev, IN Shokolenko - Gene, 1995 - Elsevier
Four mini-Tn10 derivatives bearing the nptII, cat, aacC1 and tet genes along with MCS of the
pBluescriptII plasmid were constructed. These derivatives are embedded into the γ-ori R6K …
pBluescriptII plasmid were constructed. These derivatives are embedded into the γ-ori R6K …
pBAM1: an all-synthetic genetic tool for analysis and construction of complex bacterial phenotypes
E Martínez-García, B Calles, M Arévalo-Rodríguez… - BMC microbiology, 2011 - Springer
Background Since publication in 1977 of plasmid pBR322, many breakthroughs in Biology
have depended on increasingly sophisticated vector platforms for analysis and engineering …
have depended on increasingly sophisticated vector platforms for analysis and engineering …
A mini‐Tn5‐derived transposon with reportable and selectable markers enables rapid generation and screening of insertional mutants in Gram‐negative bacteria
ES Nazareno, B Acharya… - Letters in Applied …, 2021 - academic.oup.com
We re‐engineered a classic tool for mutagenesis and gene expression studies in Gram‐
negative bacteria. Our modified Tn5‐based transposon contains multiple features that allow …
negative bacteria. Our modified Tn5‐based transposon contains multiple features that allow …
A mini-Mu transposon-based method for multiple DNA fragment integration into bacterial genomes
A method for construction of bacterial strains with multiple DNA inserted into their
chromosomes has been developed based on the mini-Mu transposon and FLP/FRT …
chromosomes has been developed based on the mini-Mu transposon and FLP/FRT …
Mini-Mu transposition of bacterial genes on the transmissible plasmid
M Weiserova, J Hubáček, V Brenner, ES Piruzian… - Folia microbiologica, 1987 - Springer
Using the pRM30 plasmid, an Ap s deletion derivative of broad host range plasmid RP4 with
integrated new miniMu 5 (11 kb), we followed the transfer of Escherichia coli chromosomal …
integrated new miniMu 5 (11 kb), we followed the transfer of Escherichia coli chromosomal …
TnpR Encoded by an ISPpu12 Isoform Regulates Transposition of Two Different ISL3-Like Insertion Sequences in Pseudomonas stutzeri after Conjugative …
JA Christie-Oleza, B Nogales, J Lalucat… - Journal of …, 2010 - Am Soc Microbiol
Pseudomonas stutzeri AN10 has two IS L3-like insertion sequences (ISs). One of them has
been recently described as IS Pst9. In this study we show that the second IS, situated 4.5 kb …
been recently described as IS Pst9. In this study we show that the second IS, situated 4.5 kb …