Analysis by LC–MS/MS of endogenous steroids from human serum, plasma, endometrium and endometriotic tissue

MR Häkkinen, T Heinosalo, N Saarinen… - … of Pharmaceutical and …, 2018 - Elsevier
Abstract An LC–MS/MS method was developed and validated to analyze simultaneously
estrogens (estradiol, E2; estrone, E1), androgens (testosterone, T; androstenedione, A4; …

Simultaneous analysis by LC–MS/MS of 22 ketosteroids with hydroxylamine derivatization and underivatized estradiol from human plasma, serum and prostate tissue

MR Häkkinen, T Murtola, R Voutilainen… - … of pharmaceutical and …, 2019 - Elsevier
This study describes a validated LC–MS/MS method for assaying 23 steroids within a single
run from 150 μl of human plasma, serum or prostatic tissue homogenate. Isotope-labeled …

An LC-MS/MS method for the simultaneous quantification of 32 steroids in human plasma

M Šimková, L Kolátorová, P Drašar, J Vítků - Journal of Chromatography B, 2022 - Elsevier
A development of robust and rapid method with simple sample preparation for the analysis
of steroids of C 18-, C 19-, C 21-families is of interest of many research groups. Here we …

Development and validation of an LC‐MS/MS method for profiling 39 urinary steroids (estrogens, androgens, corticoids, and progestins)

HH Son, WS Yun, SH Cho - Biomedical Chromatography, 2020 - Wiley Online Library
Abnormal production or metabolism of steroid hormones is responsible for the development
of endocrine diseases. Thus, accurate quantification of steroid hormones is needed for both …

Profiling intact steroid sulfates and unconjugated steroids in biological fluids by liquid chromatography-tandem mass spectrometry (LC-MS-MS)

CE Galuska, MF Hartmann, A Sánchez-Guijo… - Analyst, 2013 - pubs.rsc.org
Within the combined DFG research project “Sulfated Steroids in Reproduction” an analytical
method was needed for determining sulfated and unconjugated steroids with highest …

Novel liquid chromatography tandem mass spectrometry (LC-MS/MS) methods for measuring steroids

BG Keevil - Best practice & research Clinical endocrinology & …, 2013 - Elsevier
Liquid chromatography tandem mass spectrometry (LC-MS/MS) is increasingly becoming
the method of choice for steroid hormone measurements due to small sample volumes, fast …

[HTML][HTML] Quantitative-profiling method of serum steroid hormones by hydroxylamine-derivatization HPLC–MS

Q Liu, Q Chi, RT Fan, HD Tian, X Wang - Natural Products and …, 2019 - Springer
A sensitive and rapid high performance liquid chromatography–mass spectrometry (HPLC–
MS) method was developed and validated for simultaneous quantification of ten steroid …

A sensitive, simple and robust LC–MS/MS method for the simultaneous quantification of seven androgen-and estrogen-related steroids in postmenopausal serum

Y Ke, J Bertin, R Gonthier, JN Simard… - The Journal of steroid …, 2014 - Elsevier
Steroids were first analyzed by immunoassay-based methods followed by gas
chromatography mass spectrometry (GC–MS or GC–MS/MS) with derivatization techniques …

Fast and sensitive liquid chromatography–mass spectrometry assay for seven androgenic and progestagenic steroids in human serum

P Keski-Rahkonen, K Huhtinen, M Poutanen… - The Journal of steroid …, 2011 - Elsevier
A fast and sensitive LC–MS/MS method for the quantitative analysis of seven steroid
hormones in 150μl of human serum was developed and validated. The following …

Chemical derivatization for enhancing sensitivity during LC/ESI–MS/MS quantification of steroids in biological samples: a review

T Higashi, S Ogawa - The Journal of Steroid Biochemistry and Molecular …, 2016 - Elsevier
Sensitive and specific methods for the detection, characterization and quantification of
endogenous steroids in body fluids or tissues are necessary for the diagnosis, pathological …