LarvaSPA, a method for mounting drosophila larva for long-term time-lapse imaging

H Ji, C Han - JoVE (Journal of Visualized Experiments), 2020 - jove.com
Live imaging is a valuable approach for investigating cell biology questions. The Drosophila
larva is particularly suited for in vivo live imaging because the larval body wall and most …

In vivo imaging of intact Drosophila larvae at sub-cellular resolution

Y Zhang, P Füger, SB Hannan, JV Kern… - JoVE (Journal of …, 2010 - jove.com
Recent improvements in optical imaging, genetically encoded fluorophores and genetic
tools allowing efficient establishment of desired transgenic animal lines have enabled …

Applications of immobilization of drosophila tissues with fibrin clots for live imaging

J Januschke, N Loyer - JoVE (Journal of Visualized Experiments), 2020 - jove.com
Drosophila is an important model system to study a vast range of biological questions.
Various organs and tissues from different developmental stages of the fly such as imaginal …

[HTML][HTML] Bellymount-Pulsed Tracking: A Novel Approach for Real-Time In vivo Imaging of Drosophila Abdominal Tissues

S Balachandra, AA Amodeo - bioRxiv, 2024 - ncbi.nlm.nih.gov
Quantitative live imaging is a valuable tool that offers insights into cellular dynamics.
However, many fundamental biological processes are incompatible with current live imaging …

[HTML][HTML] An Immobilization Technique for Long-Term Time-Lapse Imaging of Explanted Drosophila Tissues

MP Bostock, AR Prasad, R Chaouni… - Frontiers in Cell and …, 2020 - frontiersin.org
Time-lapse imaging is an essential tool to study dynamic biological processes that cannot
be discerned from fixed samples alone. However, imaging cell-and tissue-level processes in …

Long-term in vivo imaging of Drosophila larvae

P Kakanj, SA Eming, L Partridge, M Leptin - Nature Protocols, 2020 - nature.com
The Drosophila larva has been used to investigate many processes in cell biology, including
morphogenesis, physiology and responses to drugs and new therapeutic compounds …

Upright imaging of Drosophila embryos

M Belu, M Javier, K Ayasoufi, S Frischmann… - JoVE (Journal of …, 2010 - jove.com
Several well-known morphogenetic gradients and cellular movements occur along the
dorsal/ventral axis of the Drosophila embryo. However, the current techniques used to view …

Using microfluidics chips for live imaging and study of injury responses in Drosophila larvae

B Mishra, M Ghannad-Rezaie, J Li, X Wang… - JoVE (Journal of …, 2014 - jove.com
Live imaging is an important technique for studying cell biological processes, however this
can be challenging in live animals. The translucent cuticle of the Drosophila larva makes it …

Preparing individual Drosophila egg chambers for live imaging

TT Weil, RM Parton, I Davis - JoVE (Journal of Visualized Experiments), 2012 - jove.com
Live cell imaging is an important technique applied to a number of Drosophila tissues used
as models to investigate topics such as axis specification, cell differentiation and …

Removal of Drosophila muscle tissue from larval fillets for immunofluorescence analysis of sensory neurons and epidermal cells

CM Tenenbaum, ER Gavis - JoVE (Journal of Visualized Experiments), 2016 - jove.com
Drosophila larval dendritic arborization (da) neurons are a popular model for investigating
mechanisms of neuronal morphogenesis. Da neurons develop in communication with the …