CHOPCHOP v3: expanding the CRISPR web toolbox beyond genome editing
The CRISPR–Cas system is a powerful genome editing tool that functions in a diverse array
of organisms and cell types. The technology was initially developed to induce targeted …
of organisms and cell types. The technology was initially developed to induce targeted …
CHOPCHOP v2: a web tool for the next generation of CRISPR genome engineering
In just 3 years CRISPR genome editing has transformed biology, and its popularity and
potency continue to grow. New CRISPR effectors and rules for locating optimum targets …
potency continue to grow. New CRISPR effectors and rules for locating optimum targets …
Methods for optimizing CRISPR-Cas9 genome editing specificity
Advances in the development of delivery, repair, and specificity strategies for the CRISPR-
Cas9 genome engineering toolbox are helping researchers understand gene function with …
Cas9 genome engineering toolbox are helping researchers understand gene function with …
Potential pitfalls of CRISPR/Cas9‐mediated genome editing
R Peng, G Lin, J Li - The FEBS journal, 2016 - Wiley Online Library
Recently, a novel technique named the clustered regularly interspaced short palindromic
repeat (CRISPR)/CRISPR‐associated protein (Cas) 9 system has been rapidly developed …
repeat (CRISPR)/CRISPR‐associated protein (Cas) 9 system has been rapidly developed …
Making the cut
J Travis - 2015 - science.org
Making the cut | Science news careers commentary Journals Science Science brought to you
byGoogle Indexer Log in science science advances science immunology science robotics …
byGoogle Indexer Log in science science advances science immunology science robotics …
Target specificity of the CRISPR-Cas9 system
X Wu, AJ Kriz, PA Sharp - Quantitative biology, 2014 - Springer
The CRISPR-Cas9 system, naturally a defense mechanism in prokaryotes, has been
repurposed as an RNA-guided DNA targeting platform. It has been widely used for genome …
repurposed as an RNA-guided DNA targeting platform. It has been widely used for genome …
The CRISPR tool kit for genome editing and beyond
M Adli - Nature communications, 2018 - nature.com
CRISPR is becoming an indispensable tool in biological research. Once known as the
bacterial immune system against invading viruses, the programmable capacity of the Cas9 …
bacterial immune system against invading viruses, the programmable capacity of the Cas9 …
Expansion of the CRISPR–Cas9 genome targeting space through the use of H1 promoter-expressed guide RNAs
V Ranganathan, K Wahlin, J Maruotti… - Nature communications, 2014 - nature.com
The repurposed CRISPR–Cas9 system has recently emerged as a revolutionary genome-
editing tool. Here we report a modification in the expression of the guide RNA (gRNA) …
editing tool. Here we report a modification in the expression of the guide RNA (gRNA) …
sgRNAcas9: a software package for designing CRISPR sgRNA and evaluating potential off-target cleavage sites
S Xie, B Shen, C Zhang, X Huang, Y Zhang - PloS one, 2014 - journals.plos.org
Although the CRISPR/Cas9/sgRNA system efficiently cleaves intracellular DNA at desired
target sites, major concerns remain on potential “off-target” cleavage that may occur …
target sites, major concerns remain on potential “off-target” cleavage that may occur …
High-throughput screens of PAM-flexible Cas9 variants for gene knockout and transcriptional modulation
A key limitation of the widely used CRISPR enzyme S. pyogenes Cas9 is the strict
requirement of an NGG protospacer-adjacent motif (PAM) at the target site. This constraint …
requirement of an NGG protospacer-adjacent motif (PAM) at the target site. This constraint …