The CRISPR–Cas system is a powerful genome editing tool that functions in a diverse array
of organisms and cell types. The technology was initially developed to induce targeted …

In just 3 years CRISPR genome editing has transformed biology, and its popularity and
potency continue to grow. New CRISPR effectors and rules for locating optimum targets …

Advances in the development of delivery, repair, and specificity strategies for the CRISPR-
Cas9 genome engineering toolbox are helping researchers understand gene function with …

Recently, a novel technique named the clustered regularly interspaced short palindromic
repeat (CRISPR)/CRISPR‐associated protein (Cas) 9 system has been rapidly developed …

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The CRISPR-Cas9 system, naturally a defense mechanism in prokaryotes, has been
repurposed as an RNA-guided DNA targeting platform. It has been widely used for genome …

CRISPR is becoming an indispensable tool in biological research. Once known as the
bacterial immune system against invading viruses, the programmable capacity of the Cas9 …

The repurposed CRISPR–Cas9 system has recently emerged as a revolutionary genome-
editing tool. Here we report a modification in the expression of the guide RNA (gRNA) …

Although the CRISPR/Cas9/sgRNA system efficiently cleaves intracellular DNA at desired
target sites, major concerns remain on potential “off-target” cleavage that may occur …

A key limitation of the widely used CRISPR enzyme S. pyogenes Cas9 is the strict
requirement of an NGG protospacer-adjacent motif (PAM) at the target site. This constraint …