Monoclonal antibodies are attractive therapeutics for treating a wide range of human
disorders due to their exquisite binding specificity and high binding affinity. However, a …

The ability of antibodies to bind to target molecules with high affinity and specificity has led
to their widespread use in diagnostic and therapeutic applications. Nevertheless, a limitation …

Protein aggregation remains a major area of focus in the production of monoclonal
antibodies. Improving the intrinsic properties of antibodies can improve manufacturability …

Many human monoclonal antibodies display poor biophysical properties, such as low
stability and a propensity to aggregate. These unfavorable tendencies can be even more …

Therapeutic proteins such as antibodies constitute the most rapidly growing class of
pharmaceuticals for use in diverse clinical settings including cancer, chronic inflammatory …

The widespread use of monoclonal antibodies for therapeutic applications has led to intense
interest in optimizing several of their natural properties (affinity, specificity, stability, solubility …

Biologics, such as antibodies and enzymes, are crucial in research, biotechnology,
diagnostics, and therapeutics. Often, biologics with suitable functionality are discovered, but …

Highlights•Specificity is a critical attribute of drug-like monoclonal antibodies.•Antibody
specificity is compromised by excess localized charge and/or hydrophobicity.•High …

Human monoclonal antibodies often display limited thermodynamic and colloidal stabilities.
This behavior hinders their production, and places limitations on the development of novel …

The success of antibody therapeutics is strongly influenced by their multifunctional nature
that couples antigen recognition mediated by their variable regions with effector functions …