Saccharomyces cerevisiae is an important model eukaryotic microorganism and widely
applied in fundamental research and the production of various chemicals. Its ability to …

Thanks to its ease of use, modularity, and scalability, the clustered regularly interspaced
short palindromic repeats (CRISPR) system has been increasingly used in the design and …

Synthetic CRISPR–Cas transcription factors enable the construction of complex gene‐
expression programs, and chemically inducible systems allow precise control over the …

Fine-tuning of gene expression is crucial for protein expression and pathway construction,
but it still faces formidable challenges due to the hierarchical gene regulation at multiple …

Genome editing has enabled broad advances and novel approaches in studies of gene
function and structure; now, emerging methods aim to precisely engineer post …

Although only 6 years old, the CRISPR system has blossomed into a tool for rapid, on-
demand genome engineering and gene regulation in Saccharomyces cerevisiae. In this …

Dynamic control of gene expression is emerging as an important strategy for controlling flux
in metabolic pathways and improving bioproduction of valuable compounds. Integrating …

Targeted modulation of transcription is necessary for understanding complex gene networks
and has great potential for medical and industrial applications. CRISPR is emerging as a …

Transcriptional regulation is central to the complex behavior of natural biological systems
and synthetic gene circuits. Platforms for the scalable, tunable, and simple modulation of …

Most classic genetic approaches utilize binary modifications that preclude the identification
of key knockdowns for essential genes or other targets that only require moderate …