Bioactive Self-Assembled Protein Nanosheets for Stem Cell-Based Biotechnologies

H Kanso - 2024 - qmro.qmul.ac.uk
Tissue and stem cell culture methods have been dominated by glass and plastic substrates
such as Tissue culture plastic. These solid substrates, although widely used, are associated …

[HTML][HTML] Long term expansion profile of mesenchymal stromal cells at protein nanosheet-stabilised bioemulsions for next generation cell culture microcarriers

L Peng, JE Gautrot - Materials Today Bio, 2021 - Elsevier
Tremendous progress in the identification, isolation and expansion of stem cells has allowed
their application in regenerative medicine and tissue engineering, and their use as …

Engineered Protein Nanosheets for the Scale up of Mesenchymal Stem Cell Culture on Bioemulsions

M Bosch-Fortea, C Nadal, A Chrysanthou, D Marciano… - bioRxiv, 2023 - biorxiv.org
The rapid progress in cell therapies and stem cell technologies requires the development of
novel bioprocessing and biomanufacturing pipelines able to cope with the scale up of cell …

Strong elastic protein nanosheets enable the culture and differentiation of induced pluripotent stem cells on microdroplets

E Mojares, C Nadal, D Hayler, H Kanso… - Advanced …, 2024 - Wiley Online Library
Advances in stem cell technologies, revolutionizing regenerative therapies and advanced in
vitro testing, require novel cell manufacturing pipelines able to cope with scale up and …

Engineering of Co-Surfactant-Free Bioactive Protein Nanosheets for the Stabilisation of Bioemulsions Enabling Adherent Cell Expansion

A Chrysanthou, M Bosch-Fortea, JE Gautrot - bioRxiv, 2022 - biorxiv.org
Bioemulsions are attractive platforms for the scalable expansion of adherent cells and stem
cells. In these systems, cell adhesion is enabled by the assembly of protein nanosheets that …

Culture of Mesenchymal Stem Cells on Nanosheets Assembled at the Surface of Liquid Microcarriers.

L Peng - 2021 - qmro.qmul.ac.uk
Mesenchymal stem cells (MSC) are one of the prevalent cell types for potential clinical utility
including cell therapies and tissue engineering. In order to meet massive demand for clinical …

Protein nanosheet engineering, bioemulsion design and application in stem cell technologies

A Chrysanthou - 2024 - qmro.qmul.ac.uk
Liquids such as aliphatic, silicone and fluorophilic oils are not normally considered as
suitable substrates for the culture of adherent stem cells. However, recent reports …

Growth of mesenchymal stem cells at the surface of silicone, mineral and plant-based oils

L Peng, C Nadal, JE Gautrot - Biomedical Materials, 2023 - iopscience.iop.org
Bioemulsions are attractive platforms for the expansion of adherent cells in bioreactors.
Their design relies on the self-assembly of protein nanosheets at liquid–liquid interfaces …

[HTML][HTML] Impact of the multiscale viscoelasticity of quasi-2D self-assembled protein networks on stem cell expansion at liquid interfaces

D Kong, L Peng, M Bosch-Fortea, A Chrysanthou… - Biomaterials, 2022 - Elsevier
Although not typically thought to sustain cell adhesion and expansion, liquid substrates have
recently been shown to support such phenotypes, providing protein nanosheets could be …

Co-surfactant-free bioactive protein nanosheets for the stabilization of bioemulsions enabling adherent cell expansion

A Chrysanthou, M Bosch-Fortea, JE Gautrot - Biomacromolecules, 2023 - ACS Publications
Bioemulsions are attractive platforms for the scalable expansion of adherent cells and stem
cells. In these systems, cell adhesion is enabled by the assembly of protein nanosheets that …