Although mammalian cell factories are now widely used in production of biomedically
important proteins they are still relatively ineffective compared with those from prokaryotic …

There is a great demand for the improvement of mammalian cell production systems such
that they can compete economically with their prokaryotic counterparts. Of a number of …

We have shown previously that an engineered form of Renilla luciferase (SRUC) can be
secreted as a functional enzyme by mammalian cells, and that fusing wild‐type Renilla …

Mammalian cell lines are currently employed as one of the main cellular factories for the
expression of recombinant protein‐based drugs. The establishment of high‐producing cell …

We present a technology for the production of target proteins using novel cell-free systems
derived from cultured human K562 cells and Chinese hamster ovary (CHO) cells. The …

Optimizing appropriate signal peptides in mammalian cell-based protein production is
crucial given that most recombinant proteins produced in mammalian cells are thought to be …

The firefly luciferase (Luc) protein and the jellyfish green fluorescent protein (GFP) are two
commonly used molecular reporters that can be detected noninvasively in living cells. The …

The promoter activity of growth hormone (GH) was continuously monitored in rat pituitary
adenoma cells (GH3) by a secreted bioluminescence reporter, Vargula hilgendorfii (Vh) …

This unit reviews the stages involved in protein production in mammalian cells using a
stable‐expression approach. Choice of cell type is discussed, as is transfection of the host …

The selection of an optimal recombinant expression system is important for successful
protein production. Here, we compared production of human interleukin-2 (hIL-2)-green …