Asymmetric synthesis of (S)-3-chloro-1-phenyl-1-propanol using Saccharomyces cerevisiae reductase with high enantioselectivity
Chloro-1-phenyl-1-propanol is used as a chiral intermediate in the synthesis of
antidepressant drugs. Various microbial reductases were expressed in Escherichia coli, and …
antidepressant drugs. Various microbial reductases were expressed in Escherichia coli, and …
Applications of whole cell biotransformations for the production of chiral alcohols
AWI Tan - 2006 - bonndoc.ulb.uni-bonn.de
Bioreduction of (2, 5)-hexanedione to highly enantiopure (5R)-hydroxyhexane-2-one (ee>
99%) with Lactobacillus kefiri DSM 20587 was investigated. Cell immobilisation with sodium …
99%) with Lactobacillus kefiri DSM 20587 was investigated. Cell immobilisation with sodium …
Asymmetric reduction of acetophenone derivatives by Lens culinaris
DA Ferreira, JC Da Costa Assuncao… - Biocatalysis and …, 2012 - Taylor & Francis
The enzymatic reduction of acetophenone derivatives has been evaluated using whole cells
from the edible plant lentil (Lens culinaris) as biocatalyst to afford chiral (R) and (S)-alcohols …
from the edible plant lentil (Lens culinaris) as biocatalyst to afford chiral (R) and (S)-alcohols …
Improvement of natural isolates of Saccharomyces cerevisiae strains for synthesis of a chiral building block using classic genetics
N Nir, M Bahalul, R Feingersch, T Katz-Ezov… - Applied microbiology …, 2008 - Springer
The asymmetric bio-reduction of 4-chloro-acetoacetic-acid-ethyl-ester to the pharmaceutical
building block (S)-4-chloro-3-hydroxybutanoate-ethyl-ester requires the utilization of an …
building block (S)-4-chloro-3-hydroxybutanoate-ethyl-ester requires the utilization of an …
Concomitant cell‐free biosynthesis of optically pure D‐(−)‐acetoin and xylitol via a novel NAD+ regeneration in two‐enzyme cascade
BACKGROUND Acetoin and xylitol are widely used as high‐value platform chemicals with
numerous potential applications. The chiral enantiomers L‐(+)‐and D‐(−)‐acetoin are used …
numerous potential applications. The chiral enantiomers L‐(+)‐and D‐(−)‐acetoin are used …
Diversity of 4-chloroacetoacetate ethyl ester-reducing enzymes in yeasts and their application to chiral alcohol synthesis
K Kita, M Kataoka, S Shimizu - Journal of bioscience and bioengineering, 1999 - Elsevier
Enzymes which reduce 4-chloroacetoacetate ethyl ester (CAAE) to (R)-or (S)-4-chloro-3-
hydroxybutanoate ethyl ester (CHBE) were investigated. Several microorganisms which can …
hydroxybutanoate ethyl ester (CHBE) were investigated. Several microorganisms which can …
Debaryomyces hansenii as a new biocatalyst in the asymmetric reduction of substituted acetophenones
E Şahin - Biocatalysis and Biotransformation, 2017 - Taylor & Francis
Chiral secondary alcohols are convenient mediator for the synthesis of biologically active
compounds and natural products. In this study fifteen yeast strains belonging to three food …
compounds and natural products. In this study fifteen yeast strains belonging to three food …
Bioreduction of α-chloroacetophenone by whole cells of marine fungi
LC Rocha, HV Ferreira, EF Pimenta, RGS Berlinck… - Biotechnology …, 2009 - Springer
The asymmetric reduction of 2-chloro-1-phenylethanone (1) by seven strains of marine fungi
was evaluated and afforded (S)-(-)-2-chloro-1-phenylethanol with, in the best case, an …
was evaluated and afforded (S)-(-)-2-chloro-1-phenylethanol with, in the best case, an …
Enhanced Asymmetric Reduction of Ethyl 3‐Oxobutyrate by Baker's Yeast via Substrate Feeding and Enzyme Inhibition
The moderate enantioselectivity of wild form baker's yeast can be considerably increased
either by using continuous feeding to maintain a low substrate concentration throughout the …
either by using continuous feeding to maintain a low substrate concentration throughout the …
Highly efficient synthesis of chiral alcohols with a novel NADH-dependent reductase from Streptomyces coelicolor
LJ Wang, CX Li, Y Ni, J Zhang, X Liu, JH Xu - Bioresource technology, 2011 - Elsevier
An NADH-dependent reductase (ScCR) from Streptomyces coelicolor was discovered by
genome mining for carbonyl reductases. ScCR was overexpressed in Escherichia coli BL21 …
genome mining for carbonyl reductases. ScCR was overexpressed in Escherichia coli BL21 …