Tag-free proteins or protein complexes represent certainly the most authentic starting points
for functional or structural studies. They can be obtained by conventional multi-step …

Guide to Protein Purification, Second Edition provides a complete update to existing
methods in the field, reflecting the enormous advances made in the last two decades. In …

Affinity tags have become powerful tools from basic biological research to structural and
functional proteomics. They were widely used to facilitate the purification and detection of …

Abstract Background Functional Genomics, the systematic characterisation of the functions
of an organism's genes, includes the study of the gene products, the proteins. Such studies …

Isolation and dissection of native multiprotein complexes is a central theme in functional
genomics. The development of the tandem affinity purification (TAP) tag has enabled an …

Recombinant proteins overexpressed in and purified from Escherichia coli contain impurities
that are toxic in biological assays. The application of affinity purification procedures is often …

Several fusion tail systems have been developed to promote efficient recovery and
purification of recombinant proteins from crude cell extracts or culture media. In these …

Tandem affinity strategies reach exceptional protein purification grades and have
considerably improved the outcome of mass spectrometry-based proteomic experiments …

The latest advances in combinatorial peptide ligand libraries, with their unique performance
in discovering low-abundance species in proteomes, are reviewed here. Explanations of …

The relative quantification of protein expression levels in different cell samples through the
utilization of stable isotope dilution has become a standard method in the field of proteomics …