Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation
High-throughput mRNA sequencing (RNA-Seq) promises simultaneous transcript discovery
and abundance estimation,,. However, this would require algorithms that are not restricted …
and abundance estimation,,. However, this would require algorithms that are not restricted …
[HTML][HTML] Transcript assembly and abundance estimation from RNA-Seq reveals thousands of new transcripts and switching among isoforms
C Trapnell, BA Williams, G Pertea, A Mortazavi… - Nature …, 2010 - ncbi.nlm.nih.gov
High-throughput mRNA sequencing (RNA-Seq) holds the promise of simultaneous transcript
discovery and abundance estimation 1-3. We introduce an algorithm for transcript assembly …
discovery and abundance estimation 1-3. We introduce an algorithm for transcript assembly …
Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks
Recent advances in high-throughput cDNA sequencing (RNA-seq) can reveal new genes
and splice variants and quantify expression genome-wide in a single assay. The volume …
and splice variants and quantify expression genome-wide in a single assay. The volume …
Defining transcribed regions using RNA-seq
Next-generation sequencing technologies are revolutionizing genomics research. It is now
possible to generate gigabase pairs of DNA sequence within a week without time …
possible to generate gigabase pairs of DNA sequence within a week without time …
[HTML][HTML] IVT-seq reveals extreme bias in RNA sequencing
NF Lahens, IH Kavakli, R Zhang, K Hayer, MB Black… - Genome biology, 2014 - Springer
Background RNA-seq is a powerful technique for identifying and quantifying transcription
and splicing events, both known and novel. However, given its recent development and the …
and splicing events, both known and novel. However, given its recent development and the …
Transcript-level expression analysis of RNA-seq experiments with HISAT, StringTie and Ballgown
High-throughput sequencing of mRNA (RNA-seq) has become the standard method for
measuring and comparing the levels of gene expression in a wide variety of species and …
measuring and comparing the levels of gene expression in a wide variety of species and …
Mapping and quantifying mammalian transcriptomes by RNA-Seq
A Mortazavi, BA Williams, K McCue, L Schaeffer… - Nature …, 2008 - nature.com
We have mapped and quantified mouse transcriptomes by deeply sequencing them and
recording how frequently each gene is represented in the sequence sample (RNA-Seq) …
recording how frequently each gene is represented in the sequence sample (RNA-Seq) …
Optimized single-nucleus transcriptional profiling by combinatorial indexing
Single-cell combinatorial indexing RNA sequencing (sci-RNA-seq) is a powerful method for
recovering gene expression data from an exponentially scalable number of individual cells …
recovering gene expression data from an exponentially scalable number of individual cells …
Differential analysis of gene regulation at transcript resolution with RNA-seq
Differential analysis of gene and transcript expression using high-throughput RNA
sequencing (RNA-seq) is complicated by several sources of measurement variability and …
sequencing (RNA-seq) is complicated by several sources of measurement variability and …
Entering the era of single-cell transcriptomics in biology and medicine
R Sandberg - Nature methods, 2014 - nature.com
Recent technical advances have enabled RNA sequencing (RNA-seq) in single cells.
Exploratory studies have already led to insights into the dynamics of differentiation, cellular …
Exploratory studies have already led to insights into the dynamics of differentiation, cellular …