Basal bodies and centrioles are conserved microtubule-based organelles the improper
assembly of which leads to a number of diseases, including ciliopathies and cancer. Tubulin …

We have used in vitro mutagenesis and gene replacement to study the function of the
nucleotide-binding domain (NBD) of γ-tubulin in Tetrahymena thermophila. In this study, we …

Thanks to recent technological advances, the ciliate Tetrahymena thermophila has emerged
as an attractive model organism for studies on the assembly of microtubular organelles in a …

Background The multi-tubulin hypothesis proposes that each tubulin isotype performs a
unique role, or subset of roles, in the universe of microtubule function (s). To test this …

Tetrahymena and Paramecium species are widely used representatives of the phylum
Ciliata. Ciliates are particularly suitable model organisms for studying the functional …

In ciliates, different microtubular structures are nucleated from diverse Microtubule
Organizing Centers (MTOCs). γ-Tubulin is a tubulin superfamily member that plays an …

We have cloned and sequenced the two β-tubulin genes of the ciliated protozoan
Tetrahymena thermophila. The two genes encode identical 443 amino acid peptides which …

* Abbreviations used in this paper: BB, basal body; cs, cold sensitive; CVP, contractile
vacuole pore; KF, kinetodesmal fiber; Mac, macronucleus; Mic, micronucleus; MT …

Doublet and triplet microtubules are essential and highly stable core structures of centrioles,
basal bodies, cilia, and flagella. In contrast to dynamic cytoplasmic microtubules, their …

The ciliated protozoan Tetrahymena thermophila contains two distinct nuclei within a single
cell—the mitotic micronucleus and the amitotic macronucleus. Although microtubules are …