Centrioles and basal bodies fascinate by their spectacular architecture, featuring an
arrangement of nine microtubule triplets into an axial symmetry, whose biogenesis relies on …

The single alpha‐tubulin gene of Tetrahymena thermophila was isolated from a genomic
library and shown to encode a single protein. Comparisons of the rates of evolution of this …

Microtubules (MTs) are organized into distinct systems essential for cell shape, movement,
intracellular transport, and division [1]. Electron crystallographic analyses [2] provide little …

Most higher eukaryotic tubulins are separated into alpha-and beta-tubulin when
electrophoresed in NaDodSO4-denaturing gels, while many lower eukaryotic tubulins are …

Background A breakthrough in the understanding of centriole assembly was provided by the
characterization of the UNI3 gene in Chlamydomonas. Deletion of this gene, found to …

Tubulin glycylation is a posttranslational modification found in cells with cilia or flagella. The
ciliate Tetrahymena has glycylation on ciliary and cortical microtubules. We showed …

Macronuclear DNA of the ciliate Tetrahymena pyriformis contains only one size class of
fragments coding for α-tubulin, αTT. We have isolated αTT from a partial plasmid library …

ABSTRACT A temperature-sensitive mutant homozygous for the recessive gene mol b in
Tetrahymena thermophila offers opportunity for studying the direction of microtubule …

A temperature-sensitive mutation was isolated that blocks cilia regeneration and arrests
growth in Tetrahymena thermophila. Protein and RNA synthesis and ATP production …

Tubulin undergoes glutamylation, a conserved posttranslational modification of poorly
understood function. We show here that in the ciliate Tetrahymena, most of the microtubule …