Base editing—the introduction of single-nucleotide variants (SNVs) into DNA or RNA in
living cells—is one of the most recent advances in the field of genome editing. As around …

Genome editing has transformed the life sciences and has exciting prospects for use in
treating genetic diseases. Our laboratory developed base editing to enable precise and …

RNA-guided programmable nucleases from CRISPR systems generate precise breaks in
DNA or RNA at specified positions. In cells, this activity can lead to changes in DNA …

RNA base editing refers to the rewriting of genetic information within an intact RNA molecule
and serves various functions, such as evasion of the endogenous immune system and …

We recently developed base editing, a genome-editing approach that enables the
programmable conversion of one base pair into another without double-stranded DNA …

Base editors have drawn considerable academic and industrial attention in recent years
because of their ability to alter single DNA bases with precision. However, the existing …

Base editors enable targeted single-nucleotide conversions in genomic DNA. Here we show
that expression levels are a bottleneck in base-editing efficiency. We optimize cytidine (BE4) …

Smithies et al.(1985) and Jasin and colleagues (1994) provided proof of concept that
homologous recombination (HR) could be applied to the treatment of human disease and …

Genome editing methods have commonly relied on the initial introduction of double-
stranded DNA breaks (DSBs), resulting in stochastic insertions, deletions, and translocations …

Genome editing with engineered endonucleases is rapidly becoming a staple method in
developmental biology studies. Engineered nucleases permit random or designed genomic …