Single and multiple gene knockouts by CRISPR–Cas9 in maize
NM Doll, LM Gilles, MF Gérentes, C Richard, J Just… - Plant Cell Reports, 2019 - Springer
Key message The analysis of 93 mutant alleles in 18 genes demonstrated that CRISPR–
Cas9 is a robust tool for targeted mutagenesis in maize, permitting efficient generation of …
Cas9 is a robust tool for targeted mutagenesis in maize, permitting efficient generation of …
Comparison of CRISPR/Cas9 expression constructs for efficient targeted mutagenesis in rice
The CRISPR/Cas9 system is an efficient tool used for genome editing in a variety of
organisms. Despite several recent reports of successful targeted mutagenesis using the …
organisms. Despite several recent reports of successful targeted mutagenesis using the …
Targeted genome modifications in soybean with CRISPR/Cas9
Background The ability to selectively alter genomic DNA sequences in vivo is a powerful tool
for basic and applied research. The CRISPR/Cas9 system precisely mutates DNA …
for basic and applied research. The CRISPR/Cas9 system precisely mutates DNA …
Genome editing in soybean with CRISPR/Cas9
J Liu, S Gunapati, NT Mihelich, AO Stec… - Plant genome editing …, 2019 - Springer
CRISPR/Cas9 mediated genome editing technology has experienced rapid advances in
recent years and has been applied to a wide variety of plant species, including soybean …
recent years and has been applied to a wide variety of plant species, including soybean …
CRISPR/Cas mutagenesis of soybean and Medicago truncatula using a new web-tool and a modified Cas9 enzyme
The CRISPR/Cas9 system is rapidly becoming the reagent of choice for targeted
mutagenesis and gene editing in crop species. There are currently intense research efforts …
mutagenesis and gene editing in crop species. There are currently intense research efforts …
Activities and specificities of CRISPR/Cas9 and Cas12a nucleases for targeted mutagenesis in maize
CRISPR/Cas9 and Cas12a (Cpf1) nucleases are two of the most powerful genome editing
tools in plants. In this work, we compared their activities by targeting maize glossy2 gene …
tools in plants. In this work, we compared their activities by targeting maize glossy2 gene …
A CRISPR/Cas9 toolkit for multiplex genome editing in plants
HL Xing, L Dong, ZP Wang, HY Zhang, CY Han, B Liu… - BMC plant biology, 2014 - Springer
Background To accelerate the application of the CRISPR/Cas9 (clustered regularly
interspaced short palindromic repeats/CRISPR-associated protein 9) system to a variety of …
interspaced short palindromic repeats/CRISPR-associated protein 9) system to a variety of …
Efficient targeted genome modification in maize using CRISPR/Cas9 system
C Feng, J Yuan, R Wang, Y Liu, JA Birchler… - Journal of Genetics and …, 2016 - Elsevier
CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 system, which is a
newly developed technology for targeted genome modification, has been successfully used …
newly developed technology for targeted genome modification, has been successfully used …
A detailed procedure for CRISPR/Cas9-mediated gene editing in Arabidopsis thaliana
The newly developed CRISPR (Clustered Regularly Interspaced Short Palindromic
Repeats)/Cas (CRISPR-associated) system has emerged as an efficient tool for genome …
Repeats)/Cas (CRISPR-associated) system has emerged as an efficient tool for genome …
An Agrobacterium‐delivered CRISPR/Cas9 system for high‐frequency targeted mutagenesis in maize
SN Char, AK Neelakandan… - Plant biotechnology …, 2017 - Wiley Online Library
CRISPR/Cas9 is a powerful genome editing tool in many organisms, including a number of
monocots and dicots. Although the design and application of CRISPR/Cas9 is simpler …
monocots and dicots. Although the design and application of CRISPR/Cas9 is simpler …