A Static Microfluidic Device for Investigating the Chemotaxis Response to Stable, Non-linear Gradients

N Sule, D Penarete-Acosta, DL Englert… - … : Methods and Protocols, 2018 - Springer
Microfluidic technology allows fast and precise measurement of chemotaxis responses to
both attractant and repellent signals. One of the major drawbacks of current microfluidic …

Analysis of flow-based microfluidic gradient generators for the study of bacterial chemotaxis

CJ Wolfram - 2015 - search.proquest.com
Chemotaxis is a phenomenon which enables cells to sense concentrations of certain
chemical species in their microenvironment and move towards chemically favorable regions …

Perspectives in flow-based microfluidic gradient generators for characterizing bacterial chemotaxis

CJ Wolfram, GW Rubloff, X Luo - Biomicrofluidics, 2016 - pubs.aip.org
Chemotaxis is a phenomenon which enables cells to sense concentrations of certain
chemical species in their microenvironment and move towards chemically favorable regions …

Diffusion-based and long-range concentration gradients of multiple chemicals for bacterial chemotaxis assays

M Kim, T Kim - Analytical Chemistry, 2010 - ACS Publications
We present a diffusion-driven and long-range concentration gradient generator that uses
hydrogel as a porous membrane to prevent convection flows but allow the diffusion of cell …

Investigation of bacterial chemotaxis using a simple three-point microfluidic system

H Kim, J Ali, K Phuyal, S Park, MJ Kim - BioChip Journal, 2015 - Springer
A three-point microfluidic system was developed and used to experimentally verify bacterial
chemotaxis with known chemoeffectors. Using pneumatically-controlled micro-valves, the …

Investigation of bacterial chemotaxis in flow-based microfluidic devices

DL Englert, MD Manson, A Jayaraman - Nature protocols, 2010 - nature.com
The plug-in-pond and capillary assays are convenient methods for measuring attractant and
repellent bacterial chemotaxis. However, these assays do not provide quantitative …

A microfluidic device for quantifying bacterial chemotaxis in stable concentration gradients

DL Englert, MD Manson, A Jayaraman - JoVE (Journal of Visualized …, 2010 - jove.com
Chemotaxis allows bacteria to approach sources of attractant chemicals or to avoid sources
of repellent chemicals. Bacteria constantly monitor the concentration of specific …

A sensitive, versatile microfluidic assay for bacterial chemotaxis

H Mao, PS Cremer, MD Manson - Proceedings of the …, 2003 - National Acad Sciences
We have developed a microfluidic assay for bacterial chemotaxis in which a gradient of
chemoeffectors is established inside a microchannel via diffusion between parallel streams …

A three-channel microfluidic device for generating static linear gradients and its application to the quantitative analysis of bacterial chemotaxis

J Diao, L Young, S Kim, EA Fogarty, SM Heilman… - Lab on a Chip, 2006 - pubs.rsc.org
We have developed a prototype three-channel microfluidic chip that is capable of generating
a linear concentration gradient within a microfluidic channel and is useful in the study of …

Bacterial chemotaxis in linear and nonlinear steady microfluidic gradients

T Ahmed, TS Shimizu, R Stocker - Nano letters, 2010 - ACS Publications
Diffusion-based microfluidic devices can generate steady, arbitrarily shaped chemical
gradients without requiring fluid flow and are ideal for studying chemotaxis of free-swimming …