Intracytoplasmic sperm injection (ICSI)-mediated gene transfer has recently been shown to
be an effective technique for producing transgenic pigs; however, the types of sperm …

Understanding the behavior of transgenes introduced into oocytes or embryos is essential
for evaluating the methodologies for transgenic animal production. We investigated the …

Intracytoplasmic sperm injection–sperm-mediated gene transfer (ICSI-SMGT) is a useful tool
for the production of transgenic mice but is still rather inefficient in farm animals. In the …

In relation to the growing recent interest in the establishment of sperm‐mediated gene
transfer (SMGT) technology as a convenient and effective method for the simple production …

The objective of this study was to examine whether the ICSI-mediated gene transfer method
using in vitro matured oocytes and frozen sperm head could actually produce transgenic …

Transgenesis constitutes an important tool for pharmacological protein production and
livestock improvement. We evaluated the potential of laparoscopic insemination (LI), in vitro …

Content The aim of the present study was to establish the technology of intracytoplasmic
sperm injection (ICSI) in rabbit by using the sperm frozen without cryoprotectants …

An efficient and low‐cost production of transgenic pigs has significant applications to the pig
industry and biomedical science. Generation of transgenic pig by sperm‐mediated gene …

Transgenesis is an essential tool in many biotechnological applications. Intracytoplasmic
sperm injection (ICSI)-mediated gene transfer is a powerful technique to obtain transgenic …

As a method of producing transgenic animals, spermatozoa have been used to fertilize
mammalian oocytes through natural copulation, artificial insemination (AI), and in vitro …