Poly (A) polymerase activity was first detected in yeast extracts, primarily in association with
the ribosomal fraction, by Twu and Bretthauer in 1971 (Twu, JS, and Bretthauer, RK.(1971) …

Yeast extracts have a poly (A) polymerase activity which catalyzes the addition of adenylate
residues to the 3′-terminus of primer RNA. On purification of this activity, two poly (A) …

Michael J. Holland,! Gordon L. Hager, § and William J. Rutter* abstract: Yeast poly (adenylic
acid)-containing messenger RNA was isolated fromtotal cellular RNA by affinity …

A polyadenylate polymerase involved in the polyadenylation of pre-mRNA has been purified
6,000-fold to apparent homogeneity from extracts of calf thymus. In the last purification step …

Publisher Summary This chapter discusses the enzymes that are likely to be responsible for
synthesis of poly (A) sequences and has led to new methods for obtaining preparations of …

Previous studies have demonstrated that poly (A) polymerase exists as “bound” and “free”
forms localized in the chromatin and nuclear supernatant (sap) fractions, respectively …

Highly purified poly (A) polymerase (polynucleotide adenylyltransferase, EC 2.7. 7.19),
which synthesizes poly (A) from ATP substrate, can also catalyze hydrolysis of poly (A). The …

Poly (A) polymerase was purified 22,000-fold to homogeneity from a whole cell extract of
Saccharomyces cerevisiae with a yield of 22%. The enzyme is a monomeric polypeptide …

cDNA clones encoding mammalian poly (A) polymerase were isolated with probes
generated by the polymerase chain reaction based on amino acid sequences derived from …

Poly (A) polymerase (EC 2.7. 7.19), the enzyme which appears to be responsible for the
polyadenylation of mRNA, has been detected in several mammalian tissues and is present …