Analysis of Pseudomonas putida growth on non‐trivial carbon sources using transcriptomics and genome‐scale modelling

I D'Arrigo, JGR Cardoso, M Rennig… - Environmental …, 2019 - Wiley Online Library
Pseudomonas putida is characterized by a versatile metabolism and stress tolerance traits
that allow the bacterium to cope with different environmental conditions. In this work, the …

High‐quality genome‐scale metabolic modelling of Pseudomonas putida highlights its broad metabolic capabilities

J Nogales, J Mueller, S Gudmundsson… - Environmental …, 2020 - Wiley Online Library
Genome‐scale reconstructions of metabolism are computational species‐specific
knowledge bases able to compute systemic metabolic properties. We present a …

Identification of conditionally essential genes for growth of Pseudomonas putida KT2440 on minimal medium through the screening of a genome‐wide mutant library

MA Molina‐Henares, J De La Torre… - Environmental …, 2010 - Wiley Online Library
In silico models for Pseudomonas putida KT2440 metabolism predict 68 genes to be
essential for growth on minimal medium. In this study a genome‐wide collection of single …

Genome-Scale Reconstruction and Analysis of the Pseudomonas putida KT2440 Metabolic Network Facilitates Applications in Biotechnology

J Puchałka, MA Oberhardt, M Godinho… - PLoS computational …, 2008 - journals.plos.org
A cornerstone of biotechnology is the use of microorganisms for the efficient production of
chemicals and the elimination of harmful waste. Pseudomonas putida is an archetype of …

Gross transcriptomic analysis of Pseudomonas putida for diagnosing environmental shifts

Á Hueso‐Gil, B Calles, GA O'Toole… - Microbial …, 2020 - Wiley Online Library
The biological regime of Pseudomonas putida (and any other bacterium) under given
environmental conditions results from the hierarchical expression of sets of genes that …

In silico-guided engineering of Pseudomonas putida towards growth under micro-oxic conditions

LFC Kampers, RGA Van Heck, S Donati… - Microbial Cell …, 2019 - Springer
Background Pseudomonas putida is a metabolically versatile, genetically accessible, and
stress-robust species with outstanding potential to be used as a workhorse for industrial …

A metabolic and physiological design study of Pseudomonas putida KT2440 capable of anaerobic respiration

LFC Kampers, JJ Koehorst, RJA van Heck… - BMC microbiology, 2021 - Springer
Background Pseudomonas putida KT2440 is a metabolically versatile, HV1-certified,
genetically accessible, and thus interesting microbial chassis for biotechnological …

A genome-scale metabolic reconstruction of Pseudomonas putida KT2440: i JN746 as a cell factory

J Nogales, BØ Palsson, I Thiele - BMC systems biology, 2008 - Springer
Background Pseudomonas putida is the best studied pollutant degradative bacteria and is
harnessed by industrial biotechnology to synthesize fine chemicals. Since the publication of …

Core and auxiliary functions of one-carbon metabolism in Pseudomonas putida exposed by a systems-level analysis of transcriptional and physiological responses

J Turlin, Ò Puiggené, S Donati, NT Wirth, PI Nikel - Msystems, 2023 - Am Soc Microbiol
The soil bacterium Pseudomonas putida is a robust biomanufacturing host that assimilates a
broad range of substrates while efficiently coping with adverse environmental conditions. P …

Multi-omics analysis unravels a segregated metabolic flux network that tunes co-utilization of sugar and aromatic carbons in Pseudomonas putida

MA Kukurugya, CM Mendonca, M Solhtalab… - Journal of Biological …, 2019 - ASBMB
Pseudomonas species thrive in different nutritional environments and can catabolize
divergent carbon substrates. These capabilities have important implications for the role of …