In contrast to the highly developed genetic modification systems available for manipulating
the mouse genome, at this time only simple gain of function modifications can be undertaken …

Domestic animals can be cloned using techniques such as embryo splitting and nuclear
transfer to produce genetically identical individuals. Although embryo splitting is limited to …

Established routes for the transfer of foreign genes into mice are described. The transfer of
these techniques to livestock species is described, and remaining problems are identified …

Until recently, precise modification of the animal genome by gene targeting was restricted to
the mouse because germline competent embryonic stem cells are not available in any other …

The last three decades witnessed a rapid advance of the application of genetic engineering
techniques for increasingly complex organisms, from single-cell microbial and eukaryotic …

The development of methods for cell‐mediated transgenesis, based on somatic cell nuclear
transfer, provides a tremendous opportunity to shape the genetic make‐up of livestock …

The technology of gene targeting through homologous recombination has been extremely
useful for elucidating gene functions in mice. The application of this technology was thought …

The amazing pace of discovery within the field of molecular biology has expanded the
understanding of as well as the potential for genetic engineering in laboratory and domestic …

The feasibility of introducing foreign genes into the genomes of cattle, goats, pigs, and
sheep has only recently been demonstrated. Studies have thus far focused on improving …

Gene transfer to animal cells was first achieved more than thirty years ago. Since then,
transformation technology has developed rapidly, resulting in a multitude of techniques for …