Results from high-throughput DNA cloning of Arabidopsis thaliana target genes using site-specific recombination

S Thao, Q Zhao, T Kimball, E Steffen… - Journal of structural and …, 2004 - Springer
Abstract The Center for Eukaryotic Structural Genomics (CESG) was founded as a
collaborative effort to develop technologies for the rapid and economic determination of …

Functional genomics and structural biology in the definition of gene function

M Hrmova, GB Fincher - Plant Genomics: Methods and Protocols, 2009 - Springer
Summary By mid-2007, the three-dimensional (3D) structures of some 45,000 proteins have
been solved, over a period where the linear structures of millions of genes have been …

Comparison of cell‐based and cell‐free protocols for producing target proteins from the Arabidopsis thaliana genome for structural studies

RC Tyler, DJ Aceti, CA Bingman… - Proteins: Structure …, 2005 - Wiley Online Library
We describe a comparative study of protein production from 96 Arabidopsis thaliana open
reading frames (ORFs) by cell‐based and cell‐free protocols. Each target was carried …

Protein folding in the plant cell

JA Miernyk - Plant Physiology, 1999 - academic.oup.com
Whole-genome sequencing projects have drastically changed the landscape of biological
research. The lexicon of contemporary biology contains a plethora of new terms, including …

High-throughput Purification and Quality Assurance of Arabidopsis thaliana Proteins for Eukaryotic Structural Genomics

WB Jeon, DJ Aceti, CA Bingman, FC Vojtik… - Journal of structural and …, 2005 - Springer
Abstract The Center for Eukaryotic Structural Genomics (CESG) has established procedures
for the purification of Arabidopsis proteins in a high-throughput mode. Recombinant proteins …

Building blocks for plant gene assembly

M Karimi, A Bleys, R Vanderhaeghen… - Plant physiology, 2007 - academic.oup.com
Abstract The MultiSite Gateway cloning system, based on site-specific recombination,
enables the assembly of multiple DNA fragments in predefined order, orientation, and frame …

Computational modeling of gene structure in Arabidopsis thaliana

V Brendel, W Zhu - Functional Genomics, 2002 - Springer
Computational gene identification by sequence inspection remains a challenging problem.
For a typical Arabidopsis thaliana gene with five exons, at least one of the exons is expected …

Further progress towards a catalogue of all Arabidopsis genes: analysis of a set of 5000 non‐redundant ESTs

R Cooke, M Raynal, M Laudié, F Grellet… - The Plant …, 1996 - Wiley Online Library
Nearly 7000 Arabidopsis thaliana‐expressed sequence tags (ESTs) from 10 cDNA libraries
have been sequenced, of which almost 5000 non‐redundant tags have been submitted to …

Progress in Arabidopsis genome sequencing and functional genomics

R Wambutt, G Murphy, G Volckaert, T Pohl… - Journal of …, 2000 - Elsevier
Arabidopsis thaliana has a relatively small genome of approximately 130 Mb containing
about 10% repetitive DNA. Genome sequencing studies reveal a gene-rich genome …

[HTML][HTML] Ancestral sequence reconstruction-An underused approach to understand the evolution of gene function in plants?

F Scossa, AR Fernie - Computational and Structural Biotechnology Journal, 2021 - Elsevier
Whilst substantial research effort has been placed on understanding the interactions of plant
proteins with their molecular partners, relatively few studies in plants-by contrast to work in …