The Bacillus subtilis Conjugative Plasmid pLS20 Encodes Two Ribbon-Helix-Helix Type Auxiliary Relaxosome Proteins That Are Essential for Conjugation

A Miguel-Arribas, JA Hao, JR Luque-Ortega… - Frontiers in …, 2017 - frontiersin.org
Bacterial conjugation is the process by which a conjugative element (CE) is transferred
horizontally from a donor to a recipient cell via a connecting pore. One of the first steps in the …

Discovery of a new family of relaxases in Firmicutes bacteria

G Ramachandran, A Miguel-Arribas, D Abia… - PLoS …, 2017 - journals.plos.org
Antibiotic resistance is a serious global problem. Antibiotic resistance genes (ARG), which
are widespread in environmental bacteria, can be transferred to pathogenic bacteria via …

Conjugative transfer can be inhibited by blocking relaxase activity within recipient cells with intrabodies

MP Garcillán‐Barcia, P Jurado… - Molecular …, 2007 - Wiley Online Library
Horizontal transfer of antibiotic resistance genes carried by conjugative plasmids poses a
serious health problem. As conjugative relaxases are transported to recipient cells during …

Conjugative DNA metabolism in Gram-negative bacteria

F De La Cruz, LS Frost, RJ Meyer… - FEMS microbiology …, 2010 - academic.oup.com
Bacterial conjugation in Gram-negative bacteria is triggered by a signal that connects the
relaxosome to the coupling protein (T4CP) and transferosome, a type IV secretion system …

Bacterial conjugation: a potential tool for genomic engineering

M Llosa, F de la Cruz - Research in Microbiology, 2005 - Elsevier
Bacterial conjugation is a mechanism for horizontal DNA transfer with potential for universal
DNA delivery. The conjugal machinery can be separated into three functional modules: the …

Site-specific integration of foreign DNA into minimal bacterial and human target sequences mediated by a conjugative relaxase

L Agúndez, C González-Prieto, C Machón, M Llosa - PloS one, 2012 - journals.plos.org
Background Bacterial conjugation is a mechanism for horizontal DNA transfer between
bacteria which requires cell to cell contact, usually mediated by self-transmissible plasmids …

Translocation through the conjugative type IV secretion system requires unfolding of its protein substrate

M Trokter, G Waksman - Journal of bacteriology, 2018 - Am Soc Microbiol
Bacterial conjugation, a mechanism of horizontal gene transfer, is the major means by which
antibiotic resistance spreads among bacteria (1, 2). Conjugative plasmids are transferred …

Relaxases and plasmid transfer in gram-negative bacteria

EL Zechner, G Moncalián, F de la Cruz - … IV secretion in gram-negative and …, 2017 - Springer
All plasmids that spread by conjugative transfer encode a relaxase. That includes plasmids
that encode the type IV secretion machinery necessary to mediate cell to cell transfer, as …

DNA processing reactions in bacterial conjugation

E Lanka, BM Wilkins - Annual review of biochemistry, 1995 - annualreviews.org
Bacterial conjugation is an important source of genetic plasticity. The initiation complex for
conjugative transfer of transmissible plasmids—the relaxosome—is a specific DNA-protein …

TcpM: a novel relaxase that mediates transfer of large conjugative plasmids from Clostridium perfringens

JA Wisniewski, DA Traore, TL Bannam… - Molecular …, 2016 - Wiley Online Library
Conjugative transfer of toxin and antibiotic resistance plasmids in C lostridium perfringens is
mediated by the tcp conjugation locus. Surprisingly, neither a relaxase gene nor an origin of …