Background Cell display technologies (eg bacterial display) are attractive in directed
evolution as they provide the option to use flow-cytometric cell sorting for selection from …

Highlights•Autotransporters have predominantly been used for surface display on E.
coli.•For industrial applications, host bacteria with specific features are needed.•Here we …

Autotransporters constitute a large family of natural proteins that are essential for delivering
many types of proteins and peptides across the outer membrane in Gram-negative bacteria …

Cell surface display of proteins can be used for several biotechnological applications such
as the screening of protein libraries, whole cell biocatalysis and live vaccine development …

Expressing proteins of interest as fusions to proteins of the bacterial envelope is a powerful
technique with many biotechnological and medical applications. Autotransporters have …

Escherichia coli is still a very popular host for the production of recombinant proteins at an
analytical or industrial scale. Secretion of the proteins into the culture medium or display at …

Background Bacterial surface display is of interest in many applications, including live
vaccine development, screening of protein libraries and the development of whole cell …

Background The self-sufficient autotransporter (AT) pathway, ubiquitous in Gram-negative
bacteria, combines a relatively simple protein secretion mechanism with a high transport …

Development of new affinity proteins using combinatorial protein engineering is today
established for generation of monoclonal antibodies and also essential for discovery of …

Abstract Background The Autotransporter pathway, ubiquitous in Gram-negative bacteria,
allows the efficient secretion of large passenger proteins via a relatively simple mechanism …