Recombinant DNA technology enables the production of valuable foreign proteins at high
expression rates and high concentrations. Escherichia coli is one of the most widely used …

Escherichia coli has been most widely used for production of the recombinant proteins. Over-
expression of the recombinant proteins is the mainspring of the inclusion bodies formation …

Expression of heterologous proteins in E. coli often leads to the formation of protein
aggregates known as inclusion bodies (IBs). Inclusion body aggregates pose a major hurdle …

The formation of inclusion bodies (IBs) is considered as an Achilles heel of heterologous
protein expression in bacterial hosts. Wide array of techniques has been developed to …

Recombinant proteins are widely used in industry (pharmaceutical, chemical, food,
cosmetics…), research and diagnostics; therefore, interest in their production is increasing …

Inclusion bodies produced in Escherichia coli are composed of densely packed denatured
protein molecules in the form of particles. Refolding of inclusion body proteins into bioactive …

Formation of inclusion bodies in bacterial hosts poses a major challenge for large scale
recovery of bioactive proteins. The process of obtaining bioactive protein from inclusion …

Expression of recombinant proteins in Escherichia coli is normally accompanied by the
formation of inclusion bodies (IBs). To obtain the protein product in an active (native) soluble …

Escherichia coli is widely used for the expression of recombinant proteins that do not require
glycosylation for their bioactivity (1, 2). Many commercially available recombinant hormones …

Since recombinant proteins are widely used in industry and in research, the need for their
low-cost production is increasing. Escherichia coli is one of the best known and most often …