In-vivo-generated fusion promoters in Pseudomonas putida

A Nurk, A Tamm, R Hôrak, M Kivisaar - Gene, 1993 - Elsevier
Plasmid pEST1463 carrying the promoterless pheBA operon was cloned into Pseudomonas
putida PaW85, and phenol-utilizing colonies were isolated on minimal plates containing …

Differential DNA bending introduced by the Pseudomonas putida LysR‐type regulator, CatR, at the plasmid‐borne pheBA and chromosomal catBC promoters

MR Parsek, M Kivisaar… - Molecular …, 1995 - Wiley Online Library
The plasmid‐borne pheBA operon of Pseudomonas putida strain PaW85 allows growth of
the host cells on phenol. The promoter of this operon is activated by the chromosomally …

Regulation of the catechol 1, 2-dioxygenase-and phenol monooxygenase-encoding pheBA operon in Pseudomonas putida PaW85

L Kasak, R Hôrak, A Nurk, K Talvik… - Journal of …, 1993 - Am Soc Microbiol
In Pseudomonas putida PaW85, the ortho-cleavage pathway is used for catechol
degradation. The 11.4-kb XhoI fragment cloned from phenol degradation plasmid pEST1226 …

Selection of independent plasmids determining phenol degradation inPseudomonas putida and the cloning and expression of genes encoding phenol …

M Kivisaar, R Ho, L Kasak, A Heinaru, J Habicht - Plasmid, 1990 - Elsevier
Long-term cultivation of thePseudomonas putida multiplasmid strain EST1020 on phenol
resulted in the formation of individual PHE plasmids determining phenol degradation. Four …

Organization of the horizontally transferred pheBA operon and its adjacent genes in the genomes of eight indigenous Pseudomonas strains

M Peters, A Tomikas, A Nurk - Plasmid, 2004 - Elsevier
Horizontal transfer of genes encoding phenol degradation (pheBA) in the environment has
been previously described. Complete or partial phe-operon was redetected in plasmids of …

Growth medium composition-determined regulatory mechanisms are superimposed on CatR-mediated transcription from the pheBA and catBCA promoters in …

A Tover, EL Ojangu, M Kivisaar - Microbiology, 2001 - microbiologyresearch.org
Expression of the phenol degradation pathway in Pseudomonas putida strain PaW85
requires coordinated transcription of the plasmid-borne pheBA operon encoding catechol 1 …

Integration host factor suppresses promiscuous activation of the sigma 54-dependent promoter Pu of Pseudomonas putida.

J Pérez-Martín, V De Lorenzo - Proceedings of the National …, 1995 - National Acad Sciences
In the presence of m-xylene, the Pu promoter of the TOL plasmid of Pseudomonas putida is
activated by the prokaryotic enhancer-binding protein XylR. The intervening DNA segment …

Cell density‐dependent auto‐inducible promoters for expression of recombinant proteins in Pseudomonas putida

A Meyers, C Furtmann, K Gesing… - Microbial …, 2019 - Wiley Online Library
Inducible promoters such as Plac are of limited usability for industrial protein production with
Pseudomonas putida. We therefore utilized cell density‐dependent auto‐inducible …

Transcription initiation at multiple promoters of the pfl gene by E sigma 70-dependent transcription in vitro and heterologous expression in Pseudomonas putida in …

G Sawers, AF Wagner, A Böck - Journal of bacteriology, 1989 - Am Soc Microbiol
In vitro transcription experiments were used to provide further evidence that the gene
encoding pyruvate formate-lyase (EC 2.3. 1.54) from Escherichia coli is transcribed from …

Identification and characterization of IS 1411, a new insertion sequence which causes transcriptional activation of the phenol degradation genes in Pseudomonas …

A Kallastu, R Hõrak, M Kivisaar - Journal of bacteriology, 1998 - Am Soc Microbiol
ABSTRACT A new insertion sequence (IS element), IS 1411, was identified downstream of
the phenol degradation genes pheBA that originated from plasmid DNA of Pseudomonas …