An agarose-based microfluidic platform with a gradient buffer for 3D chemotaxis studies

U Haessler, Y Kalinin, MA Swartz, M Wu - Biomedical microdevices, 2009 - Springer
The current state-of-art in 3D microfluidic chemotaxis device (μFCD) is limited by the
inherent coupling of the fluid flow and chemical concentration gradients. Here, we present …

A Cautionary Perspective on Hydrogel-Induced Concentration Gradient Generation for Studying Chemotaxis

A Sapre, R Bhattacharyya, A Sen - ACS Applied Materials & …, 2024 - ACS Publications
The achievement of consistent and static chemical gradients is critically important in the
study of diffusion and chemotaxis at the micro-and nanoscales. In this context, a number of …

[图书][B] Engineering applications of microbial chemotaxis

MT Widman - 1997 - search.proquest.com
Chemotaxis is the ability of an organism to move in response to gradients of chemicals
called chemoattractants. Many species of bacteria are known to exhibit chemotaxis to a wide …

Microfluidics for mammalian cell chemotaxis

BJ Kim, M Wu - Annals of biomedical engineering, 2012 - Springer
The emerging field of micro-technology has opened up new possibilities for exploring
cellular chemotaxis in real space and time, and at single cell resolution. Chemotactic cells …

A model of strongly biased chemotaxis reveals the trade-offs of different bacterial migration strategies

RN Bearon, WM Durham - … Medicine and Biology: A Journal of …, 2020 - academic.oup.com
Many bacteria actively bias their motility towards more favourable nutrient environments. In
liquid, cells rotate their corkscrew-shaped flagella to swim, but in surface attached biofilms …

Relationship between cellular response and behavioral variability in bacterial chemotaxis

T Emonet, P Cluzel - … of the National Academy of Sciences, 2008 - National Acad Sciences
Over the last decades, bacterial chemotaxis in Escherichia coli has emerged as a canonical
system for the study of signal transduction. A remarkable feature of this system is the …

A parallel diffusion-based microfluidic device for bacterial chemotaxis analysis

G Si, W Yang, S Bi, C Luo, Q Ouyang - Lab on a chip, 2012 - pubs.rsc.org
We developed a multiple-channel microfluidic device for bacterial chemotaxis detection.
Some characteristics such as easy operation, parallel sample adding design and fast result …

The microfluidic palette: a diffusive gradient generator with spatio-temporal control

J Atencia, J Morrow, LE Locascio - Lab on a Chip, 2009 - pubs.rsc.org
This paper describes a new microfluidic device called the “microfluidic palette”, capable of
generating multiple spatial chemical gradients simultaneously inside a microfluidic chamber …

Quantification of bacterial chemotaxis responses at the mouths of hydrogel capillaries

BA Webb, TD Arapov, BE Scharf - Bacterial Chemosensing: Methods and …, 2018 - Springer
Many chemotaxis assays allow for the assessment of bacterial chemotaxis by determining
the number of cells migrating toward a chemoattractant or away from a chemorepellent …

[HTML][HTML] Microfluidic devices fitted with “flowver” paper pumps generate steady, tunable gradients for extended observation of chemotactic cell migration

SA Baldwin, SM Van Bruggen, JM Koelbl… - …, 2021 - pubs.aip.org
Microfluidics approaches have gained popularity in the field of directed cell migration,
enabling control of the extracellular environment and integration with live-cell microscopy; …