Phenotypic Variability Shapes Bacterial Responses to Opposing Gradients

L Li, X Zhang, Y Sun, Q Ouyang, Y Tu, C Luo - PRX Life, 2024 - APS
Although bacterial chemotaxis behavior in a single gradient has been well studied,
chemotaxis of bacterial population in complex environments is not well understood. Here …

Microfluidic device for analyzing preferential chemotaxis and chemoreceptor sensitivity of bacterial cells toward carbon sources

M Kim, SH Kim, SK Lee, T Kim - Analyst, 2011 - pubs.rsc.org
We present a novel microfluidic device that enables high sensitive analyses of the
chemotactic response of motile bacterial cells (Escherichia coli) that swim toward a preferred …

Rapid and accurate generation of various concentration gradients using polydimethylsiloxane-sealed hydrogel device

M Kim, M Jia, Y Kim, T Kim - Microfluidics and nanofluidics, 2014 - Springer
We report a microfluidic device that can rapidly and accurately generate various
concentration gradients in a controllable manner for the chemotaxis study of motile bacterial …

T cell chemotaxis in a simple microfluidic device

F Lin, EC Butcher - Lab on a Chip, 2006 - pubs.rsc.org
This paper describes the use of a simple microfluidic device for studying T cell chemotaxis.
The microfluidic device is fabricated in poly (dimethylsiloxane)(PDMS) using soft-lithography …

Non‐genetic diversity modulates population performance

AJ Waite, NW Frankel, YS Dufour… - Molecular systems …, 2016 - embopress.org
Biological functions are typically performed by groups of cells that express predominantly
the same genes, yet display a continuum of phenotypes. While it is known how one …

Eukaryotic chemotaxis

WJ Rappel, WF Loomis - Wiley Interdisciplinary Reviews …, 2009 - Wiley Online Library
During eukaryotic chemotaxis, external chemical gradients guide the crawling motion of
cells. This process plays an important role in a large variety of biological systems and has …

A diffusion based long-range and steady chemical gradient generator on a microfluidic device for studying bacterial chemotaxis

N Murugesan, S Singha, T Panda… - … of Micromechanics and …, 2016 - iopscience.iop.org
Studies on chemotaxis in microfluidics device have become a major area of research to
generate physiologically similar environment in vitro. In this work, a novel micro-fluidic …

Recent developments in microfluidics-based chemotaxis studies

J Wu, X Wu, F Lin - Lab on a Chip, 2013 - pubs.rsc.org
Microfluidic devices can better control cellular microenvironments compared to conventional
cell migration assays. Over the past few years, microfluidics-based chemotaxis studies …

Open access microfluidic device for the study of cell migration during chemotaxis

D Jowhar, G Wright, PC Samson, JP Wikswo… - Integrative …, 2010 - academic.oup.com
Cells sense and interpret chemical gradients, and respond by localized responses that lead
to directed migration. An open microfluidic device (OMD) was developed to provide …

Generating controlled, dynamic chemical landscapes to study microbial behavior

F Carrara, DR Brumley, AM Hein, Y Yawata… - JoVE (Journal of …, 2020 - jove.com
We demonstrate a method for the generation of controlled, dynamic chemical
pulses―where localized chemoattractant becomes suddenly available at the microscale―to …